Ricinus communis agglutinin I functionalisation of poly(methyl methacrylate) (PMMA) as a substrate for microfluidic device

	Ricinus communis agglutinin I functionalisation of poly(methyl methacrylate) (PMMA) as a substrate for microfluidic device
	Zhu DR ; Liu X ; Wang ZX
刊名	science china-chemistry
	2012
卷号	55 期号:4 页码:537-542
关键词	CELL-CULTURE BINDING GLYCOSYLATION IMMUNOASSAYS INHIBITORS
ISSN号	1674-7291
通讯作者	wang zx
中文摘要	we report a functionalisation strategy which is able to generate ricinus communis agglutinin i (rca 120) modified pmma microfluidic device for binding and culturing living cells. the functionalisation is achieved by standard amine-aldehyde (schiff base) reaction through the cross-linker, glutaraldehyde. to prove the ability of the rca 120 modified pmma surface, the pc 12 cell line (rat pheochromocytoma cells) has been captured and cultured by the microfluidic device. in the presence of tunicamycin, the dose/time-dependence on decreasing of binding affinity of rca 120 modified device with pc 12 cell is also observed. the experimental results demonstrate that the lectin-functionalized microfluidic device can be employed as efficient cell culturing platform, and has a great promise of being used as a powerful tool for monitoring the interaction of drug with living cell.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000303184300011
公开日期	2013-10-09
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48839]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhu DR,Liu X,Wang ZX. Ricinus communis agglutinin I functionalisation of poly(methyl methacrylate) (PMMA) as a substrate for microfluidic device[J]. science china-chemistry,2012,55(4):537-542.
APA	Zhu DR,Liu X,&Wang ZX.(2012).Ricinus communis agglutinin I functionalisation of poly(methyl methacrylate) (PMMA) as a substrate for microfluidic device.science china-chemistry,55(4),537-542.
MLA	Zhu DR,et al."Ricinus communis agglutinin I functionalisation of poly(methyl methacrylate) (PMMA) as a substrate for microfluidic device".science china-chemistry 55.4(2012):537-542.