Desorption electrospray ionization (DESI) source coupling ion mobility mass spectrometry for imaging fluoropezil (DC20) distribution in rat brain

doi:10.1007/s00216-021-03563-6

	Desorption electrospray ionization (DESI) source coupling ion mobility mass spectrometry for imaging fluoropezil (DC20) distribution in rat brain
	Guo, Runcong 1,2; Zhou, Lei 1; Chen, Xiaoyan 1,2
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2021-08-18
页码	13
关键词	DESI Mass spectrometry imaging Fluoropezil (DC20) Brain distribution Optimization strategy
ISSN号	1618-2642
DOI	10.1007/s00216-021-03563-6
通讯作者	Chen, Xiaoyan(xychen@simm.ac.cn)
英文摘要	Fluoropezil (DC20) is a new selective acetylcholinesterase inhibitor, and it was developed for the treatment of Alzheimer's disease patients. In this study, a desorption electrospray ionization source coupling ion mobility mass spectrometry imaging (DESI/IMS-MSI) method was developed to explore the distribution of DC20 in brain tissue following oral administration. Rat brain coronal slices obtained 1 h and 3 h following drug dosing were used in the study. D6-DC20 was used as internal standard and sprayed by matrix sprayer on the brain slices to calibrate the matrix effect. Ion mobility separation was used to reduce the interference from background noise and the biological matrix. By optimizing DESI-MSI parameters for improved sensitivity, the limit of quantitation of the method was 1.45 pg/mm(2) with a linear range from 1.45 to 72.7 pg/mm(2). DESI-MSI data showed that DC20 could quickly enter and diffuse across whole brain and tended to be much more enriched in striatum than cerebral cortex and hippocampus, which was consistent with quantitative analysis using high-performance liquid chromatography-electrospray tandem mass spectrometry to measure DC20 concentration in each homogenized brain sub-region. The workflow of tissue imaging method optimization and strategy were established, and for the first time, the DESI-MSI technique and optimized method were used to explore the distribution characteristics of DC20 in rat brain, which could help elucidate pharmacological effect mechanisms and improve clinical outcomes.
资助项目	National Natural Science Foundation of China[82003841] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2021281]
WOS关键词	TISSUE ; QUANTIFICATION ; NEUROTRANSMITTERS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000685716900001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/298110]
专题	中国科学院上海药物研究所
通讯作者	Chen, Xiaoyan
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, 501 Haike Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Guo, Runcong,Zhou, Lei,Chen, Xiaoyan. Desorption electrospray ionization (DESI) source coupling ion mobility mass spectrometry for imaging fluoropezil (DC20) distribution in rat brain[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2021:13.
APA	Guo, Runcong,Zhou, Lei,&Chen, Xiaoyan.(2021).Desorption electrospray ionization (DESI) source coupling ion mobility mass spectrometry for imaging fluoropezil (DC20) distribution in rat brain.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,13.
MLA	Guo, Runcong,et al."Desorption electrospray ionization (DESI) source coupling ion mobility mass spectrometry for imaging fluoropezil (DC20) distribution in rat brain".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2021):13.