Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system

doi:10.1016/j.foodcont.2021.108194

	Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system
	Wang, Shujuan 1; Fan, Yiling 1; Feng, Zhen 1; Song, Minghui 1; Li, Qiongqiong 1; Jiang, Bo 1; Qin, Feng 1; Liu, Hao 1; Lan, Lefu 2; Yang, Meicheng 1
刊名	FOOD CONTROL
	2021-12-01
卷号	130 页码:8
关键词	CRISPR/Cas12a Fe3O4@PDA@UiO-66-NH2 Nucleic acid Detection Escherichia coli O157:H7
ISSN号	0956-7135
DOI	10.1016/j.foodcont.2021.108194
通讯作者	Yang, Meicheng(yangmeicheng@vip.sina.com)
英文摘要	Escherichia coli O157:H7 (E. coli O157:H7) is one of the most dangerous foodborne pathogens causing severe illnesses in the elderly, children and immunocompromised people. Traditional methods could not meet the detection requirements of high speed, sensitivity, and specificity. The clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic is a novel high sensitivity and specificity detection method. In this study, a rapid nucleic acid of Escherichia coli O157:H7 was detected using rfbE gene based on CRISPR/Cas12a system. The limit of detection for DNA and bacterial concentrations was 0.9 pg/mu L and 6.5 x 104 CFU/mL, respectively. Furthermore, when the initial bacterial inoculum was 14 CFU/mL in the ground beef sample, the target was detected after 4 h of culture through Metal Organic Framework immunomagnetic beads (Fe3O4@PDA@UiO-66-NH2) enrichment.
资助项目	National Key R&D Program of China[2018YFC1603900]
WOS关键词	METAL-ORGANIC FRAMEWORKS ; IN-GROUND BEEF ; DIAGNOSTICS ; ENRICHMENT
WOS研究方向	Food Science & Technology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000684874200009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/298002]
专题	中国科学院上海药物研究所
通讯作者	Yang, Meicheng
作者单位	1.Shanghai Inst Food & Drug Control, NMPA Key Lab Testing Technol Pharmaceut Microbiol, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Shujuan,Fan, Yiling,Feng, Zhen,et al. Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system[J]. FOOD CONTROL,2021,130:8.
APA	Wang, Shujuan.,Fan, Yiling.,Feng, Zhen.,Song, Minghui.,Li, Qiongqiong.,...&Yang, Meicheng.(2021).Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system.FOOD CONTROL,130,8.
MLA	Wang, Shujuan,et al."Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system".FOOD CONTROL 130(2021):8.