Structural characterization of two novel polysaccharides from Gastrodia elata and their effects on Akkermansia muciniphila

doi:10.1016/j.ijbiomac.2021.06.157

	Structural characterization of two novel polysaccharides from Gastrodia elata and their effects on Akkermansia muciniphila
	Huo, Jiangyan 1,2; Lei, Min 1,2; Zhou, Yang 1,2; Zhong, Xianchun 1,2; Liu, Yameng 1,2; Hou, Jinjun 1,2; Long, Huali 1,2; Zhang, Zijia 1,2; Tian, Menghua 3; Xie, Cen 1,2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2021-09-01
卷号	186 页码:501-509
关键词	Gastrodia data Akkermansia muciniphila Polysaccharide
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2021.06.157
通讯作者	Xie, Cen(xiecen@simm.ac.cn) ; Wu, Wanying(wanyingwu@simm.ac.cn)
英文摘要	Two homogeneous polysaccharides, GEP-3 and GEP-4, were purified from Gastrodia data, a precious traditional Chinese medicine. Their structural characteristics were obtained using HPGPC, PMP-HPLC, LC/MS, FT-IR, NMR, and SEM methods. GEP-3 was 1,4-glucan with molecular weight of 20 kDa. Interestingly, GEP-4 comprised of a backbone of ->[4)-alpha-Glcp-(1](10)->[4)-alpha-Glcp-(1 ->](5)[6)-beta-Glcp-(1](11)-> 6)-alpha-Glcp-(3 -> and two branches of beta-Glcp and p-hydroxybenzyl alcohol citrate, with repeating p-hydroxybenzyl alcohol attached to the backbone chain at O-6 position of -> 4,6)-alpha-Glcp-(1 -> and O-1 position of -> 3,6)-alpha-Glcp-(1 -> GEP-4 is a novel polysaccharide obtained and characterized for the first time. Bioactivity test indicated that both of them significantly promote the growth of Akkermansia muciniphila (Akk. muciniphila). Furthermore, GEP-3 and GEP-4 promoted the growth of Akk. muciniphila from high-fat diet (HFD) fecal microbiota. These results indicated that GEP-3 and GEP-4 were potential Akk. muciniphila growth promoters.
资助项目	Special Fund for the National Key Research and Development Plan of China[2018YFC1707900] ; Special Fund for the National Key Research and Development Plan of China[2018YFC1707001] ; Special Fund for the National Key Research and Development Plan of China[2018YFC1707903] ; Special Fund for the National Key Research and Development Plan of China[2018YFC1707905]
WOS关键词	RADICAL SCAVENGING ACTIVITIES ; GUT MICROBIOTA ; CONSTITUENTS ; ANTIOXIDANT ; RHIZOMES ; BL.
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000688434600006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/297701]
专题	中国科学院上海药物研究所
通讯作者	Xie, Cen; Wu, Wanying
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Zhaotong Tianma Res Inst, Zhaotong 657000, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Huo, Jiangyan,Lei, Min,Zhou, Yang,et al. Structural characterization of two novel polysaccharides from Gastrodia elata and their effects on Akkermansia muciniphila[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,186:501-509.
APA	Huo, Jiangyan.,Lei, Min.,Zhou, Yang.,Zhong, Xianchun.,Liu, Yameng.,...&Wu, Wanying.(2021).Structural characterization of two novel polysaccharides from Gastrodia elata and their effects on Akkermansia muciniphila.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,186,501-509.
MLA	Huo, Jiangyan,et al."Structural characterization of two novel polysaccharides from Gastrodia elata and their effects on Akkermansia muciniphila".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 186(2021):501-509.