Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography

doi:10.1007/s12264-021-00715-7

	Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography
	Jiang, Liyuan 1,2,3,5,6; Li, Chengjun 1,2,4,5,6; Li, Miao 1,2,4,5,6; Yin, Xianzhen 7; Wu, Tianding 1,2,4,5,6; Duan, Chunyue 1,2,4,5,6; Cao, Yong 1,2,4,5,6; Lu, Hongbin 3,4,5,6; Hu, Jianzhong 1,2,3,5,6
刊名	NEUROSCIENCE BULLETIN
	2021-06-19
页码	12
关键词	Sr mu ct 3D High-resolution Neurovascular Spinal cord
ISSN号	1673-7067
DOI	10.1007/s12264-021-00715-7
通讯作者	Cao, Yong(caoyong1912@163.com) ; Hu, Jianzhong(jianzhonghu@hotmail.com)
英文摘要	Effective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases. However, ideal techniques for simultaneously imaging neurovascular structure in a broad region of a specimen are still lacking. In this study, we combined Golgi staining with angiography and synchrotron radiation micro-computed tomography (SR mu CT) to visualize the 3D neurovascular network in the mouse spinal cord. Using our method, the 3D neurons, nerve fibers, and vasculature in a broad region could be visualized in the same image at cellular resolution without destructive sectioning. Besides, we found that the 3D morphology of neurons, nerve fiber tracts, and vasculature visualized by SR mu CT were highly consistent with that visualized using the histological method. Moreover, the 3D neurovascular structure could be quantitatively evaluated by the combined methodology. The method shown here will be useful in fundamental neuroscience studies.
资助项目	National Natural Science Foundation of China[82030071] ; National Natural Science Foundation of China[81874004] ; National Natural Science Foundation of China[81672174] ; Key R&D Program of the Hunan Provincial Science & Technology Department[2017SK2061] ; Hunan Provincial Department of Finance[(2018)2] ; Fundamental Research Funds for the Central Universities of Central South University[2018zzts254]
WOS关键词	PHASE-CONTRAST TOMOGRAPHY ; BRAIN ; HISTOLOGY ; CELLS ; CT
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000663495900001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/297128]
专题	中国科学院上海药物研究所
通讯作者	Cao, Yong; Hu, Jianzhong
作者单位	1.Cent South Univ, Xiangya Hosp, Dept Spine Surg, Changsha 410008, Peoples R China 2.Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha 410008, Peoples R China 3.Key Lab Organ Injury Aging & Regenerat Med Hunan, Changsha 410008, Peoples R China 4.Cent South Univ, Xiangya Hosp, Dept Sports Med, Changsha 410008, Peoples R China 5.Xiangya Hosp, Int Chinese Musculoskeletal Res Soc Sports Med Re, Changsha 410008, Peoples R China 6.Hunan Engn Res Ctr Sport & Hlth, Changsha 410008, Peoples R China 7.Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Liyuan,Li, Chengjun,Li, Miao,et al. Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography[J]. NEUROSCIENCE BULLETIN,2021:12.
APA	Jiang, Liyuan.,Li, Chengjun.,Li, Miao.,Yin, Xianzhen.,Wu, Tianding.,...&Hu, Jianzhong.(2021).Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography.NEUROSCIENCE BULLETIN,12.
MLA	Jiang, Liyuan,et al."Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography".NEUROSCIENCE BULLETIN (2021):12.