Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism

doi:10.1016/j.ijbiomac.2021.03.137

	Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism
	Guo, Ciliang 1,2; Wang, Yeqing 1; Zhang, Shihai 1; Zhang, Xiuqi 1,2; Du, Zhenyun 1; Li, Meixia 1; Ding, Kan 1,2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2021-06-30
卷号	181 页码:357-368
关键词	pinnatifida polysaccharide Colitis Gut microbiota Short chain fatty acids
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2021.03.137
通讯作者	Li, Meixia(limei@simm.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn)
英文摘要	Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects. Crataegus pinnatifida (Hawthorn), which is a safe medicine and food homolog plant, has been reported to prevent colitis in murine. Yet the bioactivity component and the underlying molecular mechanism remain unclear. Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1 & ndash;2 isolated from hawthorn. Our results showed HAW1 & ndash;2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1 beta, IL-6 and TNF-alpha. Meanwhile, IKK alpha/beta, IKB alpha, NF-KB and the phosphorylation levels were inhibited significantly. These findings suggested HAW1 & ndash;2 could alleviate the inflammation of colon. Further, we found the composition of gut microbiota was modified and Bacteroides including Alistipes and Odoribacter were significantly enriched. Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines. Finally, we demonstrated the anti-inflammation activity of HAW1 & ndash;2 might be induced by acetic acid. Together, the present data revealed HAW1 & ndash;2 could directly modify the gut microbiota, especially for Bacteroides, and generate SCFAs to inhibit colitis. It also implies microbiota-directed intervention in IBD patients should be particularly given more attention. (c) 2021 Published by Elsevier B.V.
资助项目	Key New Drug Creation and Manufacturing Program[2019ZX09735001] ; National Key Research and Development Program of China, Ministry of Science and Technology[2019YFC1711-000] ; National Natural Science Foundation of China[31800673]
WOS关键词	CHAIN FATTY-ACIDS ; INFLAMMATORY-BOWEL-DISEASE ; PANCREATIC CANCER ACTIVITY ; SODIUM-INDUCED COLITIS ; INTESTINAL BARRIER ; CROHNS-DISEASE ; DIETARY FIBER ; IN-VIVO ; BUTYRATE ; BLOCKING
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000656912700003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/297057]
专题	中国科学院上海药物研究所
通讯作者	Li, Meixia; Ding, Kan
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Guo, Ciliang,Wang, Yeqing,Zhang, Shihai,et al. Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,181:357-368.
APA	Guo, Ciliang.,Wang, Yeqing.,Zhang, Shihai.,Zhang, Xiuqi.,Du, Zhenyun.,...&Ding, Kan.(2021).Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,181,357-368.
MLA	Guo, Ciliang,et al."Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 181(2021):357-368.