Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation

doi:10.1038/s41522-021-00228-1

	Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
	Yao, Mingfei 1; Lu, Yanmeng 1; Zhang, Ting 2; Xie, Jiaojiao 1; Han, Shengyi 1; Zhang, Shuobo 1; Fei, Yiqiu 1; Ling, Zongxin 1; Wu, Jingjing 1; Hu, Yue 3
刊名	NPJ BIOFILMS AND MICROBIOMES
	2021-07-09
卷号	7 期号:1 页码:10
DOI	10.1038/s41522-021-00228-1
通讯作者	Li, Lanjuan(ljli@zju.edu.cn)
英文摘要	The low viability during gastrointestinal transit and poor mucoadhesion considerably limits the effectiveness of Ligilactobacillus salivarius Li01 (Li01) in regulating gut microbiota and alleviating inflammatory bowel disease (IBD). In this study, a delivery system was designed through layer-by-layer (LbL) encapsulating a single Li01cell with chitosan and alginate. The layers were strengthened by cross-linking to form a firm and mucoadhesive shell (similar to 10 nm thickness) covering the bacterial cell. The LbL Li01 displayed improved viability under simulated gastrointestinal conditions and mucoadhesive function. Almost no cells could be detected among the free Li01 after 2 h incubation in digestive fluids, while for LbL Li01, the total reduction was around 3 log CFU/mL and the viable number of cells remained above 6 log CFU/mL. Besides, a 5-fold increase in the value of rupture length and a two-fold increase in the number of peaks were found in the (bacteria-mucin) adhesion curves of LbL Li01, compared to those of free Li01. Oral administration with LbL Li01 on colitis mice facilitated intestinal barrier recovery and restoration of the gut microbiota. The improved functionality of Li01 by LbL encapsulation could increase the potential for the probiotic to be used in clinical applications to treat IBD; this should be explored in future studies.
资助项目	National Key Research and Development Program of China[2018YFC2000500] ; National Natural Science Foundation of China[32001683] ; Beijing Advanced Innovation Center for Food Nutrition and Human Health ; National Science Foundation of China[81790631]
WOS关键词	IN-VITRO ; GUT MICROBIOTA ; PROBIOTICS ; DELIVERY ; POLYSACCHARIDES ; VISCOSITY ; PROTEINS ; BACTERIA ; ADHESION ; COLITIS
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	NATURE RESEARCH
WOS记录号	WOS:000674446800001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/296744]
专题	中国科学院上海药物研究所
通讯作者	Li, Lanjuan
作者单位	1.Zhejiang Univ, Natl Clin Res Ctr Infect Dis,Affiliated Hosp 1, Coll Med,State Key Lab Diag & Treatment Infect Di, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Peoples R China 2.Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Bone Marrow, Hangzhou, Peoples R China 3.Zhejiang Univ, Coll Comp Sci & Technol, Hangzhou, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Mol Imaging Res, Shanghai, Peoples R China 5.Linkoping Univ, Dept Biomed & Clin Sci, Linkoping, Sweden
推荐引用方式 GB/T 7714	Yao, Mingfei,Lu, Yanmeng,Zhang, Ting,et al. Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation[J]. NPJ BIOFILMS AND MICROBIOMES,2021,7(1):10.
APA	Yao, Mingfei.,Lu, Yanmeng.,Zhang, Ting.,Xie, Jiaojiao.,Han, Shengyi.,...&Li, Lanjuan.(2021).Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation.NPJ BIOFILMS AND MICROBIOMES,7(1),10.
MLA	Yao, Mingfei,et al."Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation".NPJ BIOFILMS AND MICROBIOMES 7.1(2021):10.