Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula

doi:10.3390/ijms21144968

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 2012年后新成立研究组

	Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula
	Guo, Shiqi 2; Zhang, Xiaojia 2; Bai, Quanzi 2; Zhao, Weiyue 2; Fang, Yuegenwang 2; Zhou, Shaoli 2; Zhao, Baolin ; He, Liangliang 2; Chen, Jianghua
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2020
卷号	21 期号:14 页码:-
关键词	dwarfism gene cloning MNP1 CPS Medicago truncatula
ISSN号	1422-0067
DOI	10.3390/ijms21144968
英文摘要	Plant height is a vital agronomic trait that greatly determines crop yields because of the close relationship between plant height and lodging resistance. Legumes play a unique role in the worldwide agriculture; however, little attention has been given to the molecular basis of their height. Here, we characterized the first dwarf mutantmini plant 1(mnp1) of the model legume plantMedicago truncatula. Our study found that both cell length and the cell number of internodes were reduced in amnp1mutant. Using the forward genetic screening and subsequent whole-genome resequencing approach, we cloned theMNP1gene and found that it encodes a putative copalyl diphosphate synthase (CPS) implicated in the first step of gibberellin (GA) biosynthesis. MNP1 was highly homologous toPisum sativumLS. The subcellular localization showed that MNP1 was located in the chloroplast. Further analysis indicated that GA(3)could significantly restore the plant height ofmnp1-1, and expression ofMNP1in acps1mutant ofArabidopsispartially rescued its mini-plant phenotype, indicating the conservation function of MNP1 in GA biosynthesis. Our results provide valuable information for understanding the genetic regulation of plant height inM. truncatula.
学科主题	Biochemistry & Molecular Biology ; Chemistry
语种	英语
WOS记录号	WOS:000554084400001
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/11793]
专题	西双版纳热带植物园_2012年后新成立研究组
作者单位	1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Us, Xishuangbanna Trop Bot Garden, 88 Xuefu Rd, Kunming 650223, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Guo, Shiqi,Zhang, Xiaojia,Bai, Quanzi,et al. Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(14):-.
APA	Guo, Shiqi.,Zhang, Xiaojia.,Bai, Quanzi.,Zhao, Weiyue.,Fang, Yuegenwang.,...&Chen, Jianghua.(2020).Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(14),-.
MLA	Guo, Shiqi,et al."Cloning and Functional Analysis of Dwarf GeneMini Plant 1(MNP1) inMedicago truncatula".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.14(2020):-.