Effective removal of antibiotic resistance genes and potential links with archaeal communities during vacuum-type composting and positive-pressure composting

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Effective removal of antibiotic resistance genes and potential links with archaeal communities during vacuum-type composting and positive-pressure composting
	Fan, Haonan; Wu, Shanghua; Woodley, John; Zhuang, Guoqiang; Bai, Zhihui; Xu, Shengjun; Wang, Xuan; Zhuang, Xuliang
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2020-03
卷号	89 页码:277-286
关键词	Antibiotic resistance genes (ARGs) Composting Archaea Human pathogenic bacteria Bacterial communities
ISSN号	1001-0742
英文摘要	As a major reservoir of antibiotics, animal manure contributes a lot to the augmented environmental pressure of antibiotic resistance genes (ARGs). This might be the first study to explore the effects of different ventilation types on the control of ARGs and to identify the relationships between archaeal communities and ARGs during the composting of dairy manure. Several ARGs were quantified via Real-time qPCR and microbial communities including bacteria and archaea were analyzed by High-throughput sequencing during vacuum-type composting (VTC) and positive-pressure composting (PPC). The total detected ARGs and class I integrase gene (intI1) under VTC were significantly lower than that under PPC during each stage of the composting (p < 0.001). The relative abundance of potential human pathogenic bacteria (HPB) which were identified based on sequencing information and correlation analysis decreased by 74.6% and 91.4% at the end of PPC and VTC, respectively. The composition of archaeal communities indicated that methane-producing archaea including Methanobrevibacter, Methanocorpusculum and Methanosphaera were dominant throughout the composting. Redundancy analysis suggested that Methanobrevibacter and Methanocorpusculum were positively correlated with all of the detected ARGs. Network analysis determined that the possible hosts of ARGs were different under VTC and PPC, and provided new sights about potential links between archaea and ARGs. Our results showed better performance of VTC in reducing ARGs and potential HPB and demonstrated that some archaea could also be influential hosts of ARGs, and caution the risks of archaea carrying ARGs. (c) 2019 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/45158]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Fan, Haonan,Wu, Shanghua,Woodley, John,et al. Effective removal of antibiotic resistance genes and potential links with archaeal communities during vacuum-type composting and positive-pressure composting[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2020,89:277-286.
APA	Fan, Haonan.,Wu, Shanghua.,Woodley, John.,Zhuang, Guoqiang.,Bai, Zhihui.,...&Zhuang, Xuliang.(2020).Effective removal of antibiotic resistance genes and potential links with archaeal communities during vacuum-type composting and positive-pressure composting.JOURNAL OF ENVIRONMENTAL SCIENCES,89,277-286.
MLA	Fan, Haonan,et al."Effective removal of antibiotic resistance genes and potential links with archaeal communities during vacuum-type composting and positive-pressure composting".JOURNAL OF ENVIRONMENTAL SCIENCES 89(2020):277-286.