Tracking Mercury in Individual Tetrahymena Using a Capillary Single-Cell Inductively Coupled Plasma Mass Spectrometry Online System

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Tracking Mercury in Individual Tetrahymena Using a Capillary Single-Cell Inductively Coupled Plasma Mass Spectrometry Online System
	Shi, Jianbo; Ji, Xiaomeng; Wu, Qi; Liu, Hongwei; Qu, Guangbo; Yin, Yongguang; Hu, Ligang; Jiang, Guibin
刊名	ANALYTICAL CHEMISTRY
	2020-01-07
卷号	92 期号:1 页码:622-627
ISSN号	0003-2700
英文摘要	As a kind of unicellular eukaryotic protozoa, Tetrahymena is located at the bottom of the aquatic food webs and plays an essential role in the bioaccumulation of mercury (Hg). To track Hg in individual Tetrahymena, a capillary single-cell inductively coupled plasma mass spectrometry (ICPMS) online system was developed. The experimental and instrumental conditions were optimized to ensure the signal detected was the Hg uptake in individual Tetrahymena. Moreover, a quantitative method was established and validated by detecting Hg2+ standard solutions. The limit of quantity was calculated to be approximately 3.8 X 10(-15) g Hg/cell, and the detection limit for Hg2+ exposure of Tetrahymena was 0.05 mu g/L. By using the proposed method, we found the peak became wider with increasing of exposure concentrations, indicating the accumulated Hg by different Tetrahymena varied greatly, and the difference was more significant at higher exposure concentration. This novel method has the advantages of high sensitivity and real-time detection in individual Tetrahymena, and it could be widely used for further tracking the accumulation of mercury and other metals at the single cell level.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44832]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Shi, Jianbo,Ji, Xiaomeng,Wu, Qi,et al. Tracking Mercury in Individual Tetrahymena Using a Capillary Single-Cell Inductively Coupled Plasma Mass Spectrometry Online System[J]. ANALYTICAL CHEMISTRY,2020,92(1):622-627.
APA	Shi, Jianbo.,Ji, Xiaomeng.,Wu, Qi.,Liu, Hongwei.,Qu, Guangbo.,...&Jiang, Guibin.(2020).Tracking Mercury in Individual Tetrahymena Using a Capillary Single-Cell Inductively Coupled Plasma Mass Spectrometry Online System.ANALYTICAL CHEMISTRY,92(1),622-627.
MLA	Shi, Jianbo,et al."Tracking Mercury in Individual Tetrahymena Using a Capillary Single-Cell Inductively Coupled Plasma Mass Spectrometry Online System".ANALYTICAL CHEMISTRY 92.1(2020):622-627.