An electroosmotic flow-free two-direction migration strategy enables fast affinity capillary electrophoresis to study the weak interactions between basic peptides and RNA

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	An electroosmotic flow-free two-direction migration strategy enables fast affinity capillary electrophoresis to study the weak interactions between basic peptides and RNA
	Yuan, Zheng; Xu, Peng; Yu, Fangzhi; Zhang, Dapeng; Zhao, Qiang; Yu, Wenqiang; Wang, Hailin
刊名	ANALYTICAL METHODS
	2020-12-28
卷号	12 期号:48 页码:5833-5838
ISSN号	1759-9660
英文摘要	Affinity Capillary Electrophoresis (ACE) is a useful analytical tool to study noncovalent interactions. However, it remains challenging for ACE to measure weak and unstable interactions due to the fast dissociation of the binding complex and the possible destruction of the complex by a high electric field. In this study, we proposed a two-direction migration strategy that enables ACE to detect weak and unstable but important interactions by decreasing the migration distance of the binding complex and controlling the opposite migration direction of the free probe. By synthesizing a polyacrylamide-coated neutral capillary, free of electroosmotic flow, two-direction CE migration of basic peptides (positively charged) and peptide-RNA complexes (negatively charged) was achieved. Furthermore, the weak interactions between small nuclear U2 RNA and histone peptides were detected by this two-direction migration CE approach. The effects of the methylation states of histone peptides on the weak peptide-RNA interactions were also explored by this new approach. Collectively, the suggested modification of the ACE method is able to qualitatively characterize weak interactions.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44597]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Yuan, Zheng,Xu, Peng,Yu, Fangzhi,et al. An electroosmotic flow-free two-direction migration strategy enables fast affinity capillary electrophoresis to study the weak interactions between basic peptides and RNA[J]. ANALYTICAL METHODS,2020,12(48):5833-5838.
APA	Yuan, Zheng.,Xu, Peng.,Yu, Fangzhi.,Zhang, Dapeng.,Zhao, Qiang.,...&Wang, Hailin.(2020).An electroosmotic flow-free two-direction migration strategy enables fast affinity capillary electrophoresis to study the weak interactions between basic peptides and RNA.ANALYTICAL METHODS,12(48),5833-5838.
MLA	Yuan, Zheng,et al."An electroosmotic flow-free two-direction migration strategy enables fast affinity capillary electrophoresis to study the weak interactions between basic peptides and RNA".ANALYTICAL METHODS 12.48(2020):5833-5838.