Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts

	Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts
	Wu, Rui 2; Wu, Zeming 1; Wang, Xianhui 2; Yang, Pengcheng 2; Yu, Dan 2; Zhao, Chunxia 1; Xu, Guowang 1; Kang, Le 2
刊名	proceedings of the national academy of sciences of the united states of america
	2012-02-28
卷号	109 期号:9 页码:3259-3263
关键词	aggregation metabolic profiling phase change
通讯作者	许国旺 ; lekang
产权排序	2,2
英文摘要	phenotypic plasticity occurs prevalently and plays a vital role in adaptive evolution. however, the underlying molecular mechanisms responsible for the expression of alternate phenotypes remain unknown. here, a density-dependent phase polyphenism of locusta migratoria was used as the study model to identify key signaling molecules regulating the expression of phenotypic plasticity. metabolomic analysis, using high-performance liquid chromatography and gas chromatography-mass spectrometry, showed that solitarious and gregarious locusts have distinct metabolic profiles in hemolymph. a total of 319 metabolites, many of which are involved in lipid metabolism, differed significantly in concentration between the phases. in addition, the time course of changes in the metabolic profiles of locust hemolymph that accompany phase transition was analyzed. carnitine and its acyl derivatives, which are involved in the lipid beta-oxidation process, were identified as key differential metabolites that display robust correlation with the time courses of phase transition. rnai silencing of two key enzymes from the carnitine system, carnitine acetyltransferase and palmitoyltransferase, resulted in a behavioral transition from the gregarious to solitarious phase and the corresponding changes of metabolic profiles. in contrast, the injection of exogenous acetylcarnitine promoted the acquisition of gregarious behavior in solitarious locusts. these results suggest that carnitines mediate locust phase transition possibly through modulating lipid metabolism and influencing the nervous system of the locusts.
学科主题	物理化学
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	acetyl-l-carnitine ; rat-brain ; behavior ; methylation ; insects ; tool
收录类别	SCI
语种	英语
WOS记录号	WOS:000300828200021
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118435]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Wu, Rui,Wu, Zeming,Wang, Xianhui,et al. Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts[J]. proceedings of the national academy of sciences of the united states of america,2012,109(9):3259-3263.
APA	Wu, Rui.,Wu, Zeming.,Wang, Xianhui.,Yang, Pengcheng.,Yu, Dan.,...&Kang, Le.(2012).Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts.proceedings of the national academy of sciences of the united states of america,109(9),3259-3263.
MLA	Wu, Rui,et al."Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts".proceedings of the national academy of sciences of the united states of america 109.9(2012):3259-3263.