Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis

	Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis
	Zhang, Zhenbin 1,2; Wang, Fangjun 1; Xu, Bo 1,2; Qin, Hongqiang 1,2; Ye, Mingliang 1; Zou, Hanfa 1
刊名	journal of chromatography a
	2012-09-21
卷号	1256 页码:136-143
关键词	Hybrid monolithic column Strong cation exchange Proteome analysis Phosphopeptide Enzyme reactor
ISSN号	0021-9673
通讯作者	邹汉法
产权排序	1,1
英文摘要	strong cation exchange (scx) chromatography is one of the most important separation modes in liquid chromatography and scx column is widely applied in high resolution separation or fractionation of various samples. in this work, a sulfonate scx hybrid monolithic column was successfully prepared by "one-pot" strategy and the hybrid monolith is well optimized to obtain homogenous structure. it was observed that this sulfonate scx hybrid monolithic column had similar to 7 times permeability (in water) and similar to 3 times sample loading capacity (tested by dipeptide gly-tyr) comparing to particulate scx column packed with commercial available material. then, it was used as trap column for fast sample loading of the enriched phosphopeptides. comparing to phosphate scx polymer monolithic column, the number of identified phosphopeptides increased similar to 19% due to the sulfonate group has higher retention strength than phosphate group for peptide cations. and the coverage of phosphoproteome obtained by sulfonate scx hybrid monolithic column is similar to particulate packed scx column, because they had identical sulfonate group to retain the peptide cations. finally, the sulfonate scx hybrid monolithic column was used as enzyme reactor for online protein digestion. comparing to particulate scx packed column, the identified peptides number increased 40% and the protein coverage increased 10%. this might be ascribed to the high porous structure and relative high surface area that elevated the digestion efficiency. (c) 2012 elsevier b.v. all rights reserved.
学科主题	物理化学
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; mass-spectrometry ; phosphoproteome analysis ; identification technology ; yeast proteome ; trap column ; separation ; electrochromatography ; peptides ; samples
收录类别	SCI
语种	英语
WOS记录号	WOS:000308624000018
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118251]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zhenbin,Wang, Fangjun,Xu, Bo,et al. Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis[J]. journal of chromatography a,2012,1256:136-143.
APA	Zhang, Zhenbin,Wang, Fangjun,Xu, Bo,Qin, Hongqiang,Ye, Mingliang,&Zou, Hanfa.(2012).Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis.journal of chromatography a,1256,136-143.
MLA	Zhang, Zhenbin,et al."Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis".journal of chromatography a 1256(2012):136-143.