Sexual dimorphism of DNA and histone methylation profiles in the gonads of the olive flounder Paralichthys olivaceus

doi:10.1007/s10695-021-00986-x

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Sexual dimorphism of DNA and histone methylation profiles in the gonads of the olive flounder Paralichthys olivaceus
	Liu, Yan 1,2; Wang, Wenxiang 1,2,3; Liang, Shaoshuai 1,2; Wang, Lijuan 1,2; Zou, Yuxia 1,2; Wu, Zhihao 1,2; Zou, Congcong 1,2,3; Wu, Qiaowan 1,2,3; You, Feng 1,2
刊名	FISH PHYSIOLOGY AND BIOCHEMISTRY
	2021-07-15
页码	12
关键词	DNA methylation Sex difference Olive flounder Paralichthys olivaceus
ISSN号	0920-1742
DOI	10.1007/s10695-021-00986-x
通讯作者	You, Feng(youfeng@qdio.ac.cn)
英文摘要	DNA methylation and histone methylation are two types of the most important epigenetic modifications. However, research on their differential expression in gonads of male and female fish is limited. In this study, we examined the characteristics of DNA methylation and tri-methylation of lysine 4 of histone H3 (H3K4me3) modification profiles in the gonads of the wild-type and meio-gynogenetic olive flounders Paralichthys olivaceus. Enzyme-linked immunosorbent assay (ELISA) analysis revealed that the global DNA methylation level was higher in the testis than in the ovary. Real-time quantitative PCR (qPCR) results indicated that maintenance DNA methyltransferase gene dnmt1 and de novo DNA methyltransferase gene dnmt3a are highly expressed in the ovary, while DNA demethyltransferase genes tets are highly expressed in the testis. The inconsistency of DNA methylation and methyltransferase genes in the gonads might associate with the differential distribution in the testis. 5-mC mainly located in the spermatids of the testis was shown with immunohistochemistry (IHC). Furtherly, dnmt3a and tets are mainly located in spermatocytes and oocytes with in situ hybridization (ISH) analysis. As for H3K4me3, total level is higher in the ovary detected with western blot assay. IHC results showed that the signals of H3K4me3 in Sertoli cells of the testis were stronger than those in spermatocytes and spermatids. Methyltransferase gene kmt2b and demethylase genes kdm5a and kdm5c also exhibit much higher expression in the testis with qPCR, and ISH stronger signals of kmt2b and kdm5s were detected in spermatocytes. These results implied that DNA methylation and H3K4me3 are involved in the flounder sex differences and gametogenesis.
资助项目	National Key R&D Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31702337] ; National Natural Science Foundation of China[31872558] ; Youth Research Fund of Marine Biology and Biotechnology Laboratory of Pilot National Laboratory for Marine Science and Technology[YQ2018NO01] ; National Postdoctoral Science Foundation[2020M672149] ; Shandong Postdoctoral Innovation Foundation[202003017] ; Qingdao Postdoctoral Science Foundation
WOS研究方向	Biochemistry & Molecular Biology ; Fisheries ; Physiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000673893700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/175791]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yan,Wang, Wenxiang,Liang, Shaoshuai,et al. Sexual dimorphism of DNA and histone methylation profiles in the gonads of the olive flounder Paralichthys olivaceus[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2021:12.
APA	Liu, Yan.,Wang, Wenxiang.,Liang, Shaoshuai.,Wang, Lijuan.,Zou, Yuxia.,...&You, Feng.(2021).Sexual dimorphism of DNA and histone methylation profiles in the gonads of the olive flounder Paralichthys olivaceus.FISH PHYSIOLOGY AND BIOCHEMISTRY,12.
MLA	Liu, Yan,et al."Sexual dimorphism of DNA and histone methylation profiles in the gonads of the olive flounder Paralichthys olivaceus".FISH PHYSIOLOGY AND BIOCHEMISTRY (2021):12.