Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis

	Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis
	Sun, Zhen; Qin, Hongqiang; Wang, Fangjun; Cheng, Kai; Dong, Mingming; Ye, Mingliang; Zou, Hanfa
刊名	analytical chemistry
	2012-10-16
卷号	84 期号:20 页码:8452-8456
ISSN号	0003-2700
通讯作者	邹汉法
产权排序	1, 1
英文摘要	incorporation of isotopic tag onto peptides via chemical labeling is a popular approach for quantitative proteomics. chemical labeling via solution based methods usually lead to a tedious process and sample loss because several sample preparation steps including buffer exchange and desalting are performed. in this study, a solid phase based labeling approach by integration of glycopeptide enrichment and stable isotope labeling on hydrazide beads was developed for relative quantification of protein glycosylation, by which enrichment, washing, labeling, and release of the glycopeptides were all performed on the hydrazide beads sequentially. this approach was proved to be accurate in quantitative glycoproteome analysis and have good linearity range with 2 orders of magnitude for quantification of glycopeptides. compared with dimethyl labeling conventionally performed in solution, the developed approach has better enrichment recovery (10-330% improvement) and high detection sensitivity in which 4296 of annotated glycosites (vs 26%) still can be quantified using only 10 mu g of four standard glycoprotein mixtures and 400 mu g of bovine serum album interference as starting sample. the applicability of the approach for quantitative glycopeptide profiling was also explored by differential analysis of glycoproteome between human normal serum and liver cancer serum.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	coded affinity tags ; mass-spectrometry ; proteome analysis ; isotope ; chromatography ; peptides
收录类别	SCI
语种	英语
WOS记录号	WOS:000309805200005
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118001]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Sun, Zhen,Qin, Hongqiang,Wang, Fangjun,et al. Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis[J]. analytical chemistry,2012,84(20):8452-8456.
APA	Sun, Zhen.,Qin, Hongqiang.,Wang, Fangjun.,Cheng, Kai.,Dong, Mingming.,...&Zou, Hanfa.(2012).Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis.analytical chemistry,84(20),8452-8456.
MLA	Sun, Zhen,et al."Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis".analytical chemistry 84.20(2012):8452-8456.