Programmable Live-Cell CRISPR Imaging with Toehold-Switch-Mediated Strand Displacement

doi:10.1002/anie.202009062

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Programmable Live-Cell CRISPR Imaging with Toehold-Switch-Mediated Strand Displacement
	Hao, YY ; Li, J ; Li, Q ; Zhang, LH ; Shi, JY ; Zhang, XL ; Aldalbahi, A ; Wang, LH ; Fan, CH ; Wang, F
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2020
卷号	59 期号:46 页码:20612-20618
关键词	LOGIC COMPUTATION DNA CAS9 ENDONUCLEASE
ISSN号	1433-7851
DOI	10.1002/anie.202009062
文献子类	期刊论文
英文摘要	The widespread application of CRISPR-Cas9 has transformed genome engineering. Nevertheless, the precision to control the targeting activity of Cas9 requires further improvement. We report a toehold-switch-based approach to engineer the conformation of single guide RNA (sgRNA) for programmable activation of Cas9. This activation circuit is responsive to multiple inputs and can regulate the conformation of the sgRNA through toehold-switch-mediated strand displacement. We demonstrate the orthogonal suppression and activation of Cas9 with orthogonal DNA inputs. Combination of toehold switches leads to a variety of intracellular Cas9 activation programs with simultaneous and orthogonal responses, through which multiple genome loci are displayed in different colors in a controllable manner. This approach provides a new route for programing CRISPR in living cells for genome imaging and engineering.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/33087]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.King Saud Univ, Dept Chem, Coll Sci, Riyadh 11451, Saudi Arabia 2.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Renji Hosp, Frontiers Sci Ctr Transformat Mol,Sch Med, Shanghai 200240, Peoples R China 3.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China 4.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Bioimaging Ctr,Zhangjiang Lab, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol, Shanghai 201800, Peoples R China 6.Southern Med Univ, Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6, Joint Res Ctr Precis Med, South Campus, Shanghai 201499, Peoples R China
推荐引用方式 GB/T 7714	Hao, YY,Li, J,Li, Q,et al. Programmable Live-Cell CRISPR Imaging with Toehold-Switch-Mediated Strand Displacement[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2020,59(46):20612-20618.
APA	Hao, YY.,Li, J.,Li, Q.,Zhang, LH.,Shi, JY.,...&Wang, F.(2020).Programmable Live-Cell CRISPR Imaging with Toehold-Switch-Mediated Strand Displacement.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,59(46),20612-20618.
MLA	Hao, YY,et al."Programmable Live-Cell CRISPR Imaging with Toehold-Switch-Mediated Strand Displacement".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 59.46(2020):20612-20618.