Direct DNA Methylation Profiling with an Electric Biosensor

doi:10.1021/acsnano.9b10085

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Direct DNA Methylation Profiling with an Electric Biosensor
	Ban, DK ; Liu, YS ; Wang, ZJ ; Ramachandran, S ; Sarkar, N ; Shi, Z ; Liu, WH ; Karkisaval, AG ; Martinez-Loran, E ; Zhang, F
刊名	ACS NANO
	2020
卷号	14 期号:6 页码:6743-6751
关键词	LABEL-FREE DETECTION CYTOSINE METHYLATION GRAPHENE PROTEIN ASSAY HYBRIDIZATION DIAGNOSTICS DYNAMICS NANORODS DISEASE
ISSN号	1936-0851
DOI	10.1021/acsnano.9b10085
文献子类	期刊论文
英文摘要	DNA methylation is one of the principal epigenetic mechanisms that control gene expression in humans, and its profiling provides critical information about health and disease. Current profiling methods require chemical modification of bases followed by sequencing, which is expensive and time-consuming. Here, we report a direct and rapid determination of DNA methylation using an electric biosensor. The device consists of a DNA-tweezer probe integrated on a graphene field-effect transistor for label-free, highly sensitive, and specific methylation profiling. The device performance was evaluated with a target DNA that harbors a sequence of the methylguanine-DNA methyltransferase, a promoter of glioblastoma multiforme, a lethal brain tumor. The results show that we successfully profiled the methylated and nonmethylated forms at picomolar concentrations. Further, fluorescence kinetics and molecular dynamics simulations revealed that the position of the methylation site(s), their proximity, and accessibility to the toe-hold region of the tweezer probe are the primary determinants of the device performance.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32792]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Univ Calif San Diego, Inst Engn Med, La Jolla, CA 92093 USA 2.Univ Calif San Diego, Dept Mech & Aerosp Engn & Mat Sci & Engn, La Jolla, CA 92093 USA 3.Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA 4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China 5.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Sch Chem & Chem Engn,Frontiers Sci Ctr Transforma, Shanghai 200240, Peoples R China 6.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China 7.Univ Calif San Diego, Dept Mech & Aerosp Engn, Dept Bioengn Mat Sci & Engn, La Jolla, CA 92093 USA 8.Univ Calif San Diego, Dept Mech & Aerosp Engn, La Jolla, CA 92093 USA 9.Inner Mongolia Agr Univ, Sch Life Sci, Hohhot 010018, Peoples R China 10.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA (1
推荐引用方式 GB/T 7714	Ban, DK,Liu, YS,Wang, ZJ,et al. Direct DNA Methylation Profiling with an Electric Biosensor[J]. ACS NANO,2020,14(6):6743-6751.
APA	Ban, DK.,Liu, YS.,Wang, ZJ.,Ramachandran, S.,Sarkar, N.,...&Lal, R.(2020).Direct DNA Methylation Profiling with an Electric Biosensor.ACS NANO,14(6),6743-6751.
MLA	Ban, DK,et al."Direct DNA Methylation Profiling with an Electric Biosensor".ACS NANO 14.6(2020):6743-6751.