Crystal structure of mature myroilysin and implication for its activation mechanism

doi:10.1016/j.ijbiomac.2019.11.205

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Crystal structure of mature myroilysin and implication for its activation mechanism
	Ran, TT ; Li, WD ; Sun, B ; Xu, MX ; Qiu, SS ; Xu, DQ ; He, JH ; Wang, WW
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2020
卷号	156 页码:1556-1564
关键词	ASTACIN FAMILY METALLOPROTEASE MYROILYSIN M12 METALLOPROTEASE ZINC
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2019.11.205
文献子类	期刊论文
英文摘要	Myroilysin is a novel bacterial member of M12A metalloproteases family with an uncommon "cysteine switch" activation mechanism and a unique "cap" structure. However, activation of pro-myroilysin is elusive. Here, mature myroilysin was obtained for structure determination by treating pro-myroilysin with trypsin. The structure of mature myroilysin showed that the active-site zinc ion of the mature protein is coordinated by three histidine residues, a water molecule, and a tyrosine residue (Tyr208) in the conserved Met-turn motif (SIMHY). The "cap" structure moves away from the active-site to leave the active cleft open; the newly formed N-terminus is deeply buried in myroilysin, and Glu151 forms a salt bridge directly with the first amino acid residue (Gly38), whereas they are far from each other in the pro-myroilysin. The mutation of Tyr208 indicates that Tyr208 plays an important role in activity of myroilysin. The proteolytic activity and thermostability of mutant E151A decreased dramatically, implying that Glu151 is not only important for catalysis, but also crucial for structural stability in myroilysin. Structural comparison also reveals differences existed between myroilysin and astadn. Our biochemical and structural data provide new insights into the activation of myroilysin and functional involvement of crucial residues Tyr208 and Glu151. (C) 2019 Elsevier B.V. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32772]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201204, Peoples R China 2.Nanjing Agr Univ, Coll Life Sci, Dept Microbiol, Nanjing 210095, Peoples R China
推荐引用方式 GB/T 7714	Ran, TT,Li, WD,Sun, B,et al. Crystal structure of mature myroilysin and implication for its activation mechanism[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,156:1556-1564.
APA	Ran, TT.,Li, WD.,Sun, B.,Xu, MX.,Qiu, SS.,...&Wang, WW.(2020).Crystal structure of mature myroilysin and implication for its activation mechanism.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,156,1556-1564.
MLA	Ran, TT,et al."Crystal structure of mature myroilysin and implication for its activation mechanism".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 156(2020):1556-1564.