A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio

doi:10.1021/acssensors.0c01745

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio
	Su, J ; Liu, WH ; Chen, SX ; Deng, WP ; Dou, YZ ; Zhao, ZH ; Li, JY ; Li, ZH ; Yin, H ; Ding, XT
刊名	ACS SENSORS
	2020
卷号	5 期号:12 页码:3979-3987
关键词	PROSTATE-SPECIFIC ANTIGEN ELECTROCHEMICAL BIOSENSORS ASCORBIC-ACID URIC-ACID CANCER HYBRIDIZATION NANOPARTICLES CONDUCTIVITY TEMPERATURE PERFORMANCE
ISSN号	2379-3694
DOI	10.1021/acssensors.0c01745
文献子类	期刊论文
英文摘要	Biosensing interface based on screen-printed carbon electrodes (SPCE) has been widely used for electrochemical biosensors in the field of medical diagnostics, food safety, and environmental monitoring. Nevertheless, SPCE always has a rough surface, which is easy to result in the disorder of nucleic acid capture probes, the nonspecific adsorption of signaling probes, the steric hindrance of target binding, and decrease in the signal-to-noise ratio and sensitivity of biosensors. So far, it still remains extremely challenging to develop high-efficiency carbon-based biosensing interfaces, especially for DNA probe-based assembly and functionalization. In this paper, we first used a specific DNA framework, DNA tetrahedron to solve the defects of the carbon interface, improving the biosensing ability of SPCE. With covalent coupling, the DNA tetrahedron could be immobilized on the carbon surface. Biosensing probe sequences extending from the DNA tetrahedron can be changed for different target molecules. We demonstrated that the improved SPCE could be applied for the detection of a variety of bioactive molecules. Typically, we designed gap hybridization, aptamer "sandwich" and aptamer competition reduction strategy for the detection of miRNA-141, thrombin, and ATP, respectively. High signal-to-noise ratio, sensitivity, and specificity were obtained for all of these kinds. Especially, the DNA tetrahedron-modified SPCE can work well with serum samples. The carbon-based DNA framework nano-bio interface would expand the use of SPCE and make electrochemical biosensors more available and valuable in clinical diagnosis.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32670]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Shanghai Jiao Tong Univ, Sch Biomed Engn, Inst Personalized Med, State Key Lab Oncogenes & Related Genes, Shanghai 200030, Peoples R China 2.Nanjing Univ Chinese Med, Dept Spine, TCM Hosp, Wuxi 214071, Jiangsu, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China 5.Chinse Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
推荐引用方式 GB/T 7714	Su, J,Liu, WH,Chen, SX,et al. A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio[J]. ACS SENSORS,2020,5(12):3979-3987.
APA	Su, J.,Liu, WH.,Chen, SX.,Deng, WP.,Dou, YZ.,...&Song, SP.(2020).A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio.ACS SENSORS,5(12),3979-3987.
MLA	Su, J,et al."A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio".ACS SENSORS 5.12(2020):3979-3987.