Molecular characterization of G-protein-coupled receptor (GPCR) and protein kinase A (PKA) cDNA in Perinereis aibuhitensis and expression during benzo(a)pyrene exposure

doi:10.7717/peerj.8044

	Molecular characterization of G-protein-coupled receptor (GPCR) and protein kinase A (PKA) cDNA in Perinereis aibuhitensis and expression during benzo(a)pyrene exposure
	Huang, Yi 2; Sun, Jia 2; Han, Ping 2; Zhao, Heling 1; Wang, Mengting 2; Zhou, Yibing 2; Yang, Dazuo 2; Zhao, Huan 2
刊名	PEERJ
	2019
卷号	7 页码:e8044
关键词	B(a)P Perinereis aibuhitensis GPCR PKA Real time PCR
ISSN号	2167-8359
DOI	10.7717/peerj.8044
产权排序	2
文献子类	Article
英文摘要	Background: G-protein-coupled receptors (GPCRs) are one of the most important molecules that transfer signals across the plasma membrane, and play central roles in physiological systems. The molecular architecture of GPCRs allows them to bind to diverse chemicals, including environmental contaminants. Methods: To investigate the effects of benzo(a)pyrene (B(a)P) on GPCR signaling, GPCR and the protein kinase A (PKA) catalytic subunit of Perinereis aibuhitensis were cloned. The expression patterns of these two genes during B(a)P exposure were determined with real-time fluorescence quantitative PCR. The PKA content in P. aibuhitensis under B(a)P exposure was examined. Results: The full-length cDNAs of PaGPCR and the PaPKA catalytic subunit were 1,514 and 2,662 nucleotides, respectively, encoding 338 and 350 amino acids, respectively. Multiple sequence alignments indicated that the deduced amino acid sequence of PaGPCR shared a low level of similarity with the orphan GPCRs of polychaetes and echinoderms, whereas PaPKA shared a high level of identify with the PKA catalytic subunits of other invertebrates. B(a)P exposure time-dependently elevated the expression of PaGPCR and PaPKA. The expression of both PaGPCR and PaPKA was also dose-dependent, except at a dose of 10 mg/L B(a)P. The PKA content in concentration group was elevated on day 4, with time prolonging the PKA content was down-regulated to control level. Discussion: These results suggested that GPCR signaling in P. aibuhitensis was involved in the polychaete's response to environmental contaminants.
学科主题	Multidisciplinary
URL标识	查看原文
WOS关键词	POLYCYCLIC AROMATIC-HYDROCARBONS ; BINDING ; DOMAIN
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PEERJ INC
WOS记录号	WOS:000498838200001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/31077]
专题	知识管理中心
通讯作者	Zhao, Huan
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, Asian Herpetol Res Editorial Off, Chengdu, Sichuan, Peoples R China 2.Dalian Ocean Univ, Key Lab Marine Bioresources Restorat & Habitat Re, Dalian, Liaoning, Peoples R China;
推荐引用方式 GB/T 7714	Huang, Yi,Sun, Jia,Han, Ping,et al. Molecular characterization of G-protein-coupled receptor (GPCR) and protein kinase A (PKA) cDNA in Perinereis aibuhitensis and expression during benzo(a)pyrene exposure[J]. PEERJ,2019,7:e8044.
APA	Huang, Yi.,Sun, Jia.,Han, Ping.,Zhao, Heling.,Wang, Mengting.,...&Zhao, Huan.(2019).Molecular characterization of G-protein-coupled receptor (GPCR) and protein kinase A (PKA) cDNA in Perinereis aibuhitensis and expression during benzo(a)pyrene exposure.PEERJ,7,e8044.
MLA	Huang, Yi,et al."Molecular characterization of G-protein-coupled receptor (GPCR) and protein kinase A (PKA) cDNA in Perinereis aibuhitensis and expression during benzo(a)pyrene exposure".PEERJ 7(2019):e8044.