Identification and differential regulation o microRNAs during thyroid hormone-dependent metamorphosis in Microhyla fissipes

doi:10.1186/s12864-018-4848-x

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 生物多样性与生态系统服务领域 > 中国科学院环境与应用微生物重点实验室

	Identification and differential regulation o microRNAs during thyroid hormone-dependent metamorphosis in Microhyla fissipes
	Liu, Lusha 2; Zhu, Wei 2; Liu, Jiongyu 2; Wang, Shouhong 1,2; Jiang, Jianping 2
刊名	BMC GENOMICS
	2018
卷号	19 页码:507
关键词	TH miRNA Metamorphosis Morphology Histology Microhyla fissipes Target gene
ISSN号	1471-2164
DOI	10.1186/s12864-018-4848-x
产权排序	1
文献子类	Article
英文摘要	Background: Anuran metamorphosis, which is obligatorily initiated and sustained by thyroid hormone (TH), is a dramatic example of extensive morphological, biochemical and cellular changes occurring during post-embryonic development Thus, it provides an ideal model to understand the actions of the hormone and molecular mechanisms underlying these developmental and apoptotic processes. In addition to transcriptional factors, microRNAs (miRNAs) play key roles in diverse biological processes via post-transcriptional repression of mRNAs. However, the possible role of miRNAs in anuran metamorphosis is not well understood. Screening and identification of TH-responding miRNAs are required to reveal the integrated regulatory mechanisms of TH during metamorphosis. Given the specific role of TRs during M. fissipes metamorphosis and the characteristics of M. fissipes as an ideal model, Illumina sequencing technology was employed to get a full scope of miRNA in M. fissipes metamorphosis treated by T3. Results: Morphological and histological analysis revealed that 24 h T3 treatment M. fissipes tadpoles resembled that at the climax of natural metamorphosis. Thus, small RNA libraries were constructed from control and 24 h T3 treatment groups. A total of 164 conserved miRNAs and 36 predicted novel miRNAs were characterized. Furthermore, 5' first and ninth nucleotides of miRNAs were significantly enriched in U in our study. In all, 21 miRNAs were differentially expressed between the T3 and control groups (p < 0.01). A total of 10,206 unigenes were identified as target genes of these differentially expressed miRNAs. KEGG pathway analysis indicated that the most overrepresented miRNA target genes were enriched in the "PI3k-Akt signaling pathway". In addition, a network associated with the TH signaling pathway provides an opportunity to further understand the complex biological processes that occur in metamorphosis. Conclusions: We identified a large number of miRNAs during M. fissipes metamorphosis, and 21 of them were differentially expressed in the two groups that represented two different metamorphic stages. These miRNAs may play important roles during metamorphosis. The study gives us clues for further studies of the mechanisms of anuran metamorphosis and provides a model to study the mechanism of TH-affected biological processes in humans.
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WOS关键词	GENE-EXPRESSION CHANGES ; STEM-CELL DEVELOPMENT ; XENOPUS-TROPICALIS ; AMPHIBIAN METAMORPHOSIS ; EPITHELIAL DEVELOPMENT ; SMALL RNAS ; RECEPTOR ; PATHWAYS ; LAEVIS ; MODEL
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BMC
WOS记录号	WOS:000436610300007
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30689]
专题	食品安全与环境治理领域_中国科学院环境与应用微生物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Liu, Lusha,Zhu, Wei,Liu, Jiongyu,et al. Identification and differential regulation o microRNAs during thyroid hormone-dependent metamorphosis in Microhyla fissipes[J]. BMC GENOMICS,2018,19:507.
APA	Liu, Lusha,Zhu, Wei,Liu, Jiongyu,Wang, Shouhong,&Jiang, Jianping.(2018).Identification and differential regulation o microRNAs during thyroid hormone-dependent metamorphosis in Microhyla fissipes.BMC GENOMICS,19,507.
MLA	Liu, Lusha,et al."Identification and differential regulation o microRNAs during thyroid hormone-dependent metamorphosis in Microhyla fissipes".BMC GENOMICS 19(2018):507.