Metabolic switch in energy metabolism mediates the sublethal effects induced by glyphosate-based herbicide on tadpoles of a farmland frog Microhyla fissipes

doi:10.1016/j.ecoenv.2019.109794

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 生物多样性与生态系统服务领域 > 中国科学院山地生态恢复与生物资源利用重点实验室

	Metabolic switch in energy metabolism mediates the sublethal effects induced by glyphosate-based herbicide on tadpoles of a farmland frog Microhyla fissipes
	Wang, Xungang 1,2; Chang, Liming 1,2; Zhao, Tian 1; Liu, Lusha 1; Zhang, Mengjie 1; Li, Cheng 1; Xie, Feng 1; Jiang, Jianping 1; Zhu, Wei 1
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2019
卷号	186 页码:109794
关键词	Energy metabolism Functional traits Glyphosate-based herbicide Growth suppression Metabolic switch Thermal preference
ISSN号	0147-6513
DOI	10.1016/j.ecoenv.2019.109794
产权排序	1
文献子类	Article
英文摘要	Glyphosate-based herbicides (GBHs) are widely-used agricultural chemicals, bringing potential detriments to aquatic organisms. Currently, our understanding of sublethal effects and underlying toxicologic mechanisms of GBHs are still limited, especially in amphibians. Here, the sublethal effects of a commercial GBH (KISSUN (R)) on tadpoles of a farmland dwelling frog, Microhyla fissipes, were investigated. The 10-d LC50 of "KISSUN (R)" GBH was 77.5 mg/L. Tadpoles exposed to 60-120 mg/L showed increased preference to higher temperature. After 10 days exposure, obvious growth suppression was observed in survived GBH-stressed tadpoles, characterized by dosage depended decrement in body mass, body width, total length, etc. GBH-stressed tadpoles also showed decreased tail length/snout-vent length ratio and smaller tail muscle fiber diameter. Comparative transcriptomics (control, 60 mg/L and 90 mg/L groups) was conducted to analyze the underlying molecular processes. GBH-stressed tadpoles showed downregulated transcription of ribosomal proteins and cytoskeleton proteins, which could explain their suppressed whole body and tail muscle growth. Moreover, GBH-stressed tadpoles showed transcriptional downregulation of carbohydrate and lipid catabolism, but upregulation of amino acid catabolism. It suggested a metabolic switch from carbohydrate and lipid to amino acid in these tadpoles. Accordingly, there was a trade-off between protein synthesis and energy production in respect to amino acid allocation, and it provided a metabolic explanation for why protein synthesis was downregulated and growth was suppressed in GBH-stressed tadpoles. In combination with existing literatures, we speculated that GBH might directly target the enzymes in carbohydrate and lipid catabolism, and this metabolic effect of GBH might be common to fish and amphibians. In conclusion, our study provided a systematic insight into the sublethal symptoms of GBH-stressed tadpoles, and a metabolic switch from carbohydrate and lipid to amino acid likely underlay some common toxic symptoms of GBHs on both fish and tadpoles.
学科主题	Environment/ecology
URL标识	查看原文
WOS关键词	RIBOSOME BIOGENESIS ; CHRONIC TOXICITY ; CELL-GROWTH ; SEX-RATIOS ; TEMPERATURE ; PESTICIDES ; EXPRESSION ; SURVIVAL ; BEHAVIOR ; ROUNDUP
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000496901100017
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30968]
专题	生物多样性与生态系统服务领域_中国科学院山地生态恢复与生物资源利用重点实验室
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliz, Ecol Restorat Biodivers Conservat Key Lab Sichuan, Chengdu 610041, Sichuan, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xungang,Chang, Liming,Zhao, Tian,et al. Metabolic switch in energy metabolism mediates the sublethal effects induced by glyphosate-based herbicide on tadpoles of a farmland frog Microhyla fissipes[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2019,186:109794.
APA	Wang, Xungang.,Chang, Liming.,Zhao, Tian.,Liu, Lusha.,Zhang, Mengjie.,...&Zhu, Wei.(2019).Metabolic switch in energy metabolism mediates the sublethal effects induced by glyphosate-based herbicide on tadpoles of a farmland frog Microhyla fissipes.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,186,109794.
MLA	Wang, Xungang,et al."Metabolic switch in energy metabolism mediates the sublethal effects induced by glyphosate-based herbicide on tadpoles of a farmland frog Microhyla fissipes".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 186(2019):109794.