Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity

doi:10.1111/omi.12253

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity
	Yang, Yan 1,2,3; Mao, Mengying 5; Lei, Lei 2; Li, Meng 2; Yin, Jiaxin 2; Ma, Xinrong 4; Tao, Xiang 4; Yang, Yingming 2; Hu, Tao 2
刊名	MOLECULAR ORAL MICROBIOLOGY
	2019
卷号	34 期号:2 页码:51-63
关键词	biofilm cariogenicity dexA exopolysaccharide Streptococcus mutans
ISSN号	2041-1006
DOI	10.1111/omi.12253
产权排序	5
文献子类	Article
英文摘要	The cariogenic pathogen Streptococcus mutans effectively utilizes dietary sucrose for the synthesis of exopolysaccharides (EPS), which act as a scaffold for its biofilm and thus contribute to its cariogenic pathogenicity. Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexA gene have received limited attention. Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutons biofilms. In this study, we identify the role of the S mutans dexA gene in dextran-dependent aggregation in biofilm formation. Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf. Interestingly, we reveal that inactivating the dexA gene may lead to elevated WSG synthesis in S mutans, which results in dysregulated cariogenicity in vivo. Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of the SmudexA mutants. The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants. Taken together, these data reveal that EPS synthesis finetuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria. These results highlight dexA targeting as a potentially effective tool in dental caries management.
学科主题	Microbiology
URL标识	查看原文
WOS关键词	EXOGENOUS DEXTRANASE ; EXPRESSION ; ELUCIDATION ; FLUORIDE
WOS研究方向	Dentistry, Oral Surgery & Medicine ; Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000462350200003
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/31159]
专题	环境治理与食品安全领域_农业生物技术研究
作者单位	1.Cent S Univ, Xiangya Stomatol Hosp, Xiangya Sch Stomatol, Changsha, Hunan, Peoples R China; 2.Sichuan Univ, West China Hosp Stomatol, Dept Prevent Dent, State Key Lab Oral Dis,Natl Clin Res Ctr Oral Dis, Chengdu, Sichuan, Peoples R China; 3.Cent S Univ, State Key Lab Powder Met, Changsha, Hunan, Peoples R China; 4.Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China 5.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Shanghai, Peoples R China;
推荐引用方式 GB/T 7714	Yang, Yan,Mao, Mengying,Lei, Lei,et al. Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity[J]. MOLECULAR ORAL MICROBIOLOGY,2019,34(2):51-63.
APA	Yang, Yan.,Mao, Mengying.,Lei, Lei.,Li, Meng.,Yin, Jiaxin.,...&Hu, Tao.(2019).Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.MOLECULAR ORAL MICROBIOLOGY,34(2),51-63.
MLA	Yang, Yan,et al."Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity".MOLECULAR ORAL MICROBIOLOGY 34.2(2019):51-63.