Monoamine oxidase B immobilized on magnetic nanoparticles for screening of the enzyme's inhibitors from herbal extracts

doi:10.1016/j.microc.2019.02.049

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Monoamine oxidase B immobilized on magnetic nanoparticles for screening of the enzyme's inhibitors from herbal extracts
	Jiang, Xiaolan 2,4; Yuan, Yuncong 2,4; Chen, Linbo 3; Liu, Yiming 1,4; Xiao, Mengwei 2,4; Hu, Yadong 4; Chun, Ze 4; Liao, Xun 4
刊名	MICROCHEMICAL JOURNAL
	2019
卷号	146 页码:1181-1189
关键词	Monoamine oxidase B Inhibitor screening Parkinson's disease Molecular docking Cortex fraxini Pericarpium granati
ISSN号	0026-265X
DOI	10.1016/j.microc.2019.02.049
产权排序	1
文献子类	Article
英文摘要	Monoamine oxidase B (MAO-B) was for the first time immobilized on magnetic nanoparticles to be used for screening of MAO-B inhibitors from herbal extracts. The immobilized enzyme was characterized with transmission electronic microscopy, Fourier transform infrared spectroscopy, thermal gravimetric analysis, and vibrating sample magnetometer. The immobilized enzyme retained 95% of the activity, while the thermostability was significantly increased. It was then used as solid phase extraction absorbent to extract MAO-B's ligands from two traditional Chinese medicinal plants, Cortex fraxini and Pericarpium granati. Calceolarioside B and ellagic acid were extracted from C. fraxini and P. granati, respectively, and both of them were found to be inhibitors of MAO-B with IC50 of 40.95 +/- 0.63 and 103.70 +/- 2.34 mu M, respectively. This is the first report that calceolarioside B inhibits MAO-B activity. Furthermore, inhibitory mechanisms of both compounds were studied by molecular docking analysis, the results of which demonstrated that the affinity of calceolarioside B with monoamine oxidase B was stronger than that of ellagic acid. The method proposed in this work is highly efficient for screening of MAO-B inhibitors from complex mixtures, showing great potential in discovering anti-Parkinson's disease compounds present in medicinal plants.
学科主题	Spectroscopy/instrumentation/analytical Sciences
URL标识	查看原文
WOS关键词	COVALENT IMMOBILIZATION ; PARKINSONS-DISEASE ; BLOOD ; ANTIOXIDANT ; STRATEGIES ; MEDICINE ; THERAPY ; LIPASE
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000463132100149
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/31130]
专题	环境治理与食品安全领域_农业生物技术研究
作者单位	1.Jackson State Univ, Dept Chem & Biochem, Jackson, MS 39217 USA; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Sichuan Univ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China; 4.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Jiang, Xiaolan,Yuan, Yuncong,Chen, Linbo,et al. Monoamine oxidase B immobilized on magnetic nanoparticles for screening of the enzyme's inhibitors from herbal extracts[J]. MICROCHEMICAL JOURNAL,2019,146:1181-1189.
APA	Jiang, Xiaolan.,Yuan, Yuncong.,Chen, Linbo.,Liu, Yiming.,Xiao, Mengwei.,...&Liao, Xun.(2019).Monoamine oxidase B immobilized on magnetic nanoparticles for screening of the enzyme's inhibitors from herbal extracts.MICROCHEMICAL JOURNAL,146,1181-1189.
MLA	Jiang, Xiaolan,et al."Monoamine oxidase B immobilized on magnetic nanoparticles for screening of the enzyme's inhibitors from herbal extracts".MICROCHEMICAL JOURNAL 146(2019):1181-1189.