Conservation and differentiation of polyphenol oxidase (PPO) gene introns in Triticum and Aegilops tauschii Coss.

doi:10.1007/s10722-019-00797-3

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Conservation and differentiation of polyphenol oxidase (PPO) gene introns in Triticum and Aegilops tauschii Coss.
	Li, Yun Fang; Wu, Yu; Zhang, Chun Yan; Zhang, Lei; Liu, Ze Hou; Zhang, Chi Hong
刊名	GENETIC RESOURCES AND CROP EVOLUTION
	2019
卷号	66 期号:7 页码:1443-1457
关键词	Polyphenol oxidase Intron Allopolyploidization Triticum urartu Triticum monococcum Aegilops taushcii
ISSN号	0925-9864
DOI	10.1007/s10722-019-00797-3
产权排序	1
文献子类	Article
英文摘要	Two genes (PPO1 and PPO2), located in the homoeologous chromosome group 2 of common wheat, play an important role in controlling the kernel polyphenol oxidase acitivity, which influence indirectly the time-dependent darkening and discoloration in Asian noodles and other wheat-based products. In the present study, intron sequences of PPO genes (PPO1 and PPO2) in Triticum urartu Tumanian ex Gandilyan, T. monococcum L. and Aegilops tauschii Coss., together with NCBI retrieved wheat PPO sequnces in different ploidy levels, were systematically investigated. The results highlighted that PPO intron sequences are conserved at lower classification levels (species/subspecies level), and the allopolyploidization process of common wheat has little effect on them. Sequence differences in PPO gene introns may occur long before the differentiation of the A and D genome of T. urartu and Ae. tauschii, respectively. It's interesting that different PPO intron regions vary into two different intron splicing rule within one same gene sequence: intron 1 is all fitted to the "GT-AG" splicing rule while intron 2 is the "GC-AG". In addition, the phylogenetic analysis showed the obvious differences existed in materials from different geographical distribution in T. monococcum ssp. aegilopoies, which suggest that some of them had close genetic relationship with T. urartu. These findings contribute to the application of PPO gene introns in favorable allele screening, functional molecular marker development, and phylogenetic analysis.
学科主题	Plant Sciences
URL标识	查看原文
WOS关键词	NONCANONICAL SPLICE SITES ; ALLELIC VARIATION ; COMMON WHEAT ; DOMESTICATION ; MARKERS ; ORIGIN ; WILD ; DIVERSITY ; CLONING ; COLOR
WOS研究方向	Agriculture ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000485315300008
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/31022]
专题	环境治理与食品安全领域_农业生物技术研究
通讯作者	Li, Yun Fang
作者单位	Chinese Acad Sci, Chengdu Inst Biol, Dept Agr & Biotechnol, Chengdu 610041, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Li, Yun Fang,Wu, Yu,Zhang, Chun Yan,et al. Conservation and differentiation of polyphenol oxidase (PPO) gene introns in Triticum and Aegilops tauschii Coss.[J]. GENETIC RESOURCES AND CROP EVOLUTION,2019,66(7):1443-1457.
APA	Li, Yun Fang,Wu, Yu,Zhang, Chun Yan,Zhang, Lei,Liu, Ze Hou,&Zhang, Chi Hong.(2019).Conservation and differentiation of polyphenol oxidase (PPO) gene introns in Triticum and Aegilops tauschii Coss..GENETIC RESOURCES AND CROP EVOLUTION,66(7),1443-1457.
MLA	Li, Yun Fang,et al."Conservation and differentiation of polyphenol oxidase (PPO) gene introns in Triticum and Aegilops tauschii Coss.".GENETIC RESOURCES AND CROP EVOLUTION 66.7(2019):1443-1457.