Genome-wide identification, phylogenetic and expression analysis of the heat shock transcription factor family in bread wheat (Triticum aestivum L.)

doi:10.1186/s12864-019-5876-x

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Genome-wide identification, phylogenetic and expression analysis of the heat shock transcription factor family in bread wheat (Triticum aestivum L.)
	Zhou, Min 1,2,3; Zheng, Shigang 2; Liu, Rong 2,3; Lu, Jing 2,3; Lu, Lu 2; Zhang, Chihong 2; Liu, Zehou 2; Luo, Congpei 2,3; Zhang, Lei 2; Yant, Levi 1
刊名	BMC GENOMICS
	2019
卷号	20 页码:505
关键词	Wheat Hsf Genome-wide analysis Expression profiles
ISSN号	1471-2164
DOI	10.1186/s12864-019-5876-x
产权排序	1
文献子类	Article
英文摘要	BackgroundEnvironmental toxicity from non-essential heavy metals such as cadmium (Cd), which is released from human activities and other environmental causes, is rapidly increasing. Wheat can accumulate high levels of Cd in edible tissues, which poses a major hazard to human health. It has been reported that heat shock transcription factor A 4a (HsfA4a) of wheat and rice conferred Cd tolerance by upregulating metallothionein gene expression. However, genome-wide identification, classification, and comparative analysis of the Hsf family in wheat is lacking. Further, because of the promising role of Hsf genes in Cd tolerance, there is need for an understanding of the expression of this family and their functions on wheat under Cd stress. Therefore, here we identify the wheat TaHsf family and to begin to understand the molecular mechanisms mediated by the Hsf family under Cd stress.ResultsWe first identified 78 putative Hsf homologs using the latest available wheat genome information, of which 38 belonged to class A, 16 to class B and 24 to class C subfamily. Then, we determined chromosome localizations, gene structures, conserved protein motifs, and phylogenetic relationships of these TaHsfs. Using RNA sequencing data over the course of development, we surveyed expression profiles of these TaHsfs during development and under different abiotic stresses to characterise the regulatory network of this family. Finally, we selected 13 TaHsf genes for expression level verification under Cd stress using qRT-PCR.ConclusionsTo our knowledge, this is the first report of the genome organization, evolutionary features and expression profiles of the wheat Hsf gene family. This work therefore lays the foundation for targeted functional analysis of wheat Hsf genes, and contributes to a better understanding of the roles and regulatory mechanism of wheat Hsfs under Cd stress.
学科主题	Biology
URL标识	查看原文
WOS关键词	INTRACELLULAR-DISTRIBUTION ; DEVELOPMENTAL REGULATION ; CADMIUM TOLERANCE ; STRESS RESPONSE ; WHOLE-GENOME ; DRAFT GENOME ; GENE ; PROTEIN ; EVOLUTION ; DUPLICATION
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BMC
WOS记录号	WOS:000472022300001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30938]
专题	环境治理与食品安全领域_农业生物技术研究
通讯作者	Wu, Yu
作者单位	1.Univ Nottingham, Sch Life Sci, Nottingham NG7 2RD, England 2.Chinese Acad Sci, Chengdu Inst Biol, 9,Sect 4 South RenMin Rd, Chengdu 610041, Sichuan, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
推荐引用方式 GB/T 7714	Zhou, Min,Zheng, Shigang,Liu, Rong,et al. Genome-wide identification, phylogenetic and expression analysis of the heat shock transcription factor family in bread wheat (Triticum aestivum L.)[J]. BMC GENOMICS,2019,20:505.
APA	Zhou, Min.,Zheng, Shigang.,Liu, Rong.,Lu, Jing.,Lu, Lu.,...&Wu, Yu.(2019).Genome-wide identification, phylogenetic and expression analysis of the heat shock transcription factor family in bread wheat (Triticum aestivum L.).BMC GENOMICS,20,505.
MLA	Zhou, Min,et al."Genome-wide identification, phylogenetic and expression analysis of the heat shock transcription factor family in bread wheat (Triticum aestivum L.)".BMC GENOMICS 20(2019):505.