Detection and Categorization of Diarrheagenic Escherichia coli with Auto-microfluidic Thin-film Chip Method

doi:10.1038/s41598-018-30765-3

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Detection and Categorization of Diarrheagenic Escherichia coli with Auto-microfluidic Thin-film Chip Method
	Yun, Zhenyu 2; Zeng, Lian 3; Huang, Weijian 1; Wu, Qi 2; Fan, Yiling 5; Zheng, Shigang 4; Peng, Liping 6; Han, Jiayu 3; Huang, Ying 3; Zhou, Hang 3
刊名	SCIENTIFIC REPORTS
	2018
卷号	8 页码:12926
ISSN号	2045-2322
DOI	10.1038/s41598-018-30765-3
产权排序	5
文献子类	Article
英文摘要	Diarrheagenic Escherichia coli (DEC) causes human diarrhea symptom in both healthy and immunocompromised individuals. An auto-microfluidic thin-film chip (AMTC) instrument integrating one-step multiplex PCR (mPCR) with reverse dot blot hybridization (RDBH) was developed for highthroughput detection of DEC. The novel mPCR method was developed by designing 14 specific primers and corresponding probes. 14 indexes including an endogenous gene (uidA) and 13 pathogenic genes (stx1, stx2, escV, ipaH, invE, estB, lt, pic, aggR, astA, bfpB, sth and stp) of DEC were detected. This one-step mPCR + RDBH approach is useful for simultaneous detection of numerous target genes in a single sample, whose specificity and availability have been confirmed on the positive control of 11 DEC strains. In addition, with 300 diarrheal stool samples being detected by this method, 21 were found to contain five major DEC strains. Compared with monoplex PCR and previous one-step mPCR approach, this method could detect ipaH and estB, and compared with current commercial kit, the relevance ratio of DEC detected by the AMTC method was increased by 1% in stool samples. Furthermore, the novel integration AMTC device could be a valuable detection tool for categorization of E. coli.
学科主题	Multidisciplinary
URL标识	查看原文
WOS关键词	POLYMERASE-CHAIN-REACTION ; BRAZILIAN CHILDREN ; MULTIPLEX PCR ; TOXIN ; ASSAY ; STRAINS ; GENE
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000442870300104
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30656]
专题	环境治理与食品安全领域_农业生物技术研究
作者单位	1.Chengdu CapitalBio Technol Co Ltd, 88 Bayi Rd, Chengdu 610041, Sichuan, Peoples R China; 2.China Natl Inst Standardizat, 4 Zhichun Rd, Beijing 100088, Peoples R China; 3.Sichuan Hua Hansan Bio Technol Co Ltd, 88 Keyuan South Rd, Chengdu 610041, Sichuan, Peoples R China; 4.Chinese Acad Sci, Chengdu Inst Biol, 9,Sect 4,Renmin South Rd, Chengdu 610041, Sichuan, Peoples R China; 5.Shanghai Inst Food & Drug Control, Shanghai 201203, Peoples R China; 6.Natl Ctr Agr Standardizat Monitoring & Researchin, 1218 Chuangxin 2nd Rd, Harbin 150028, Heilongjiang, Peoples R China
推荐引用方式 GB/T 7714	Yun, Zhenyu,Zeng, Lian,Huang, Weijian,et al. Detection and Categorization of Diarrheagenic Escherichia coli with Auto-microfluidic Thin-film Chip Method[J]. SCIENTIFIC REPORTS,2018,8:12926.
APA	Yun, Zhenyu.,Zeng, Lian.,Huang, Weijian.,Wu, Qi.,Fan, Yiling.,...&Chen, Haodong.(2018).Detection and Categorization of Diarrheagenic Escherichia coli with Auto-microfluidic Thin-film Chip Method.SCIENTIFIC REPORTS,8,12926.
MLA	Yun, Zhenyu,et al."Detection and Categorization of Diarrheagenic Escherichia coli with Auto-microfluidic Thin-film Chip Method".SCIENTIFIC REPORTS 8(2018):12926.