The Tryptophan decarboxylase 1 Gene From Aegilops variabilis No. 1 Regulate the Resistance Against Cereal Cyst Nematode by Altering the Downstream Secondary Metabolite Contents Rather Than Auxin Synthesis

doi:10.3389/fpls.2018.01297

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	The Tryptophan decarboxylase 1 Gene From Aegilops variabilis No. 1 Regulate the Resistance Against Cereal Cyst Nematode by Altering the Downstream Secondary Metabolite Contents Rather Than Auxin Synthesis
	Huang, Qiulan 1,3,4; Li, Lin 2; Zheng, Minghui(); Chen, Fang 1; Long, Hai 4; Deng, Guangbing 4; Pan, Zhifen 4; Liang, Junjun 4; Li, Qiao 4; Yu, Maoqun 4
刊名	FRONTIERS IN PLANT SCIENCE
	2018
卷号	9 页码:1297
关键词	cereal cyst nematode Aegilops variabilis No. 1 tryptophan decarboxylase secondary metabolite indole acetic acid
ISSN号	1664-462X
DOI	10.3389/fpls.2018.01297
产权排序	1
文献子类	Article
英文摘要	Cereal cyst nematode (CCN, Heterodera avenae) is a most important pathogen of wheat and causes tremendous yield loss annually over the world. Since the lack of resistance materials among wheat cultivars, identification and characterization of the resistance-related genes from the relatives of wheat is a necessary and efficient way. As a close relative of wheat with high resistance against CCN, Aegilops variabilis No. 1 is believed to be a valuable source for wheat breeding against this devastating disease. However so far, very few resistance-associated genes have been characterized from this species. In this study, we present that the tryptophan decarboxylase genes from Ae. variabilis No. 1 (AeVTDC1 and AeVTDC2) were both induced by CCN juveniles at the early stage of resistance response (30 h post-inoculation), with AeVTDC1 more sensitive to CCN infection than AeVTDC2. Silencing of AeVTDC1 led to compromised immunity to CCN with more CCN intrusion into roots; while overexpression AeVTDC1 in Nicotiana tabacum dramatically enhanced the resistance of plants by reducing the knots formed on roots. Metabolism analysis showed that the contents of secondary metabolites with activity of resistance to varied pathogens correlated with the expression level of AeVTDC1 in both Ae. variabilis No. 1 and the transgenic tobacco plants. In addition, the content of IAA was not affected by either silencing or overexpressing of AeVTDC1. Hence, our research provided AeVTDC1 a valuable target that mediates resistance to CCN and root knot nematode (RKN, Meloidogyne naasi) without influencing the auxin biosynthesis.
学科主题	Plant Sciences
URL标识	查看原文
WOS关键词	TRYPTOPHAN DECARBOXYLASE GENE ; STRICTOSIDINE SYNTHASE CDNAS ; CATHARANTHUS-ROSEUS ; CONSTITUTIVE EXPRESSION ; ANTHRANILATE SYNTHASE ; ALKALOID PRODUCTION ; NICOTIANA-TABACUM ; OVER-EXPRESSION ; WINTER-WHEAT ; RICE
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000443556100001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30652]
专题	环境治理与食品安全领域_农业生物技术研究
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China; 2.Zunyi Med Univ, Sch Basic Med Sci, Zunyi, Peoples R China 3.Sichuan Univ, Coll Life Sci, Chengdu, Sichuan, Peoples R China; 4.Chinese Acad Sci, Chengdu Inst Biol, Chengdu, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Huang, Qiulan,Li, Lin,Zheng, Minghui,et al. The Tryptophan decarboxylase 1 Gene From Aegilops variabilis No. 1 Regulate the Resistance Against Cereal Cyst Nematode by Altering the Downstream Secondary Metabolite Contents Rather Than Auxin Synthesis[J]. FRONTIERS IN PLANT SCIENCE,2018,9:1297.
APA	Huang, Qiulan.,Li, Lin.,Zheng, Minghui.,Chen, Fang.,Long, Hai.,...&Zhang, Haili.(2018).The Tryptophan decarboxylase 1 Gene From Aegilops variabilis No. 1 Regulate the Resistance Against Cereal Cyst Nematode by Altering the Downstream Secondary Metabolite Contents Rather Than Auxin Synthesis.FRONTIERS IN PLANT SCIENCE,9,1297.
MLA	Huang, Qiulan,et al."The Tryptophan decarboxylase 1 Gene From Aegilops variabilis No. 1 Regulate the Resistance Against Cereal Cyst Nematode by Altering the Downstream Secondary Metabolite Contents Rather Than Auxin Synthesis".FRONTIERS IN PLANT SCIENCE 9(2018):1297.