Improvement of n-caproic acid production with Ruminococcaceae bacterium CPB6: selection of electron acceptors and carbon sources and optimization of the culture medium

doi:10.1186/s12934-018-0946-3

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 应用与环境微生物研究

	Improvement of n-caproic acid production with Ruminococcaceae bacterium CPB6: selection of electron acceptors and carbon sources and optimization of the culture medium
	Wang, Han 2,3; Li, Xiangzhen 1,2; Wang, Yi 4; Tao, Yong 1,2; Lu, Shaowen 1,2; Zhu, Xiaoyu 1,2; Li, Daping 1,2
刊名	MICROBIAL CELL FACTORIES
	2018
卷号	17 页码:99
关键词	Medium-chain carboxylic acid n-Caproic acid (CA) Electron acceptor (EA) Chain elongation Ruminococcaceae bacterium CPB6
ISSN号	1475-2859
DOI	10.1186/s12934-018-0946-3
产权排序	1
文献子类	Article
英文摘要	Background: Global energy and resource shortages make it necessary to quest for renewable resources. n-Caproic acid (CA) production based on carboxylate platform by anaerobic fermentation is booming. Recently, a novel Ruminococcaceae bacterium CPB6 is shown to be a potential biotransformation factory for CA production from lactate-containing wastewater. However, little is known about the effects of different electron acceptors (EAs) on the fermentative products of strain CPB6, as well as the optimum medium for CA production. Results: In this study, batch experiments were performed to investigate the fermentative products of strain CPB6 in a lactate medium supplemented with different EAs and sugars. Supplementation of acetate, butyrate and sucrose dramatically increased cell growth and CA production. The addition of propionate or pentanoate resulted in the production of C5 or C7 carboxylic acid, respectively. Further, a Box-Behnken experiment was conducted to optimize the culture medium for CA production. The result indicated that a medium containing 13.30 g/L sucrose, 22.35 g/L lactate and 16.48 g/L butyrate supported high-titer CA production (16.73 g/L) with a maximum productivity of 6.50 g/L/day. Conclusions: This study demonstrated that strain CPB6 could produce C6-C7 carboxylic acids from lactate (as electron donor) with C2-C5 short-chain carboxylic acids (as EAs), but CA (C6 carboxylic acid) was the most major and potential product. Butyrate and sucrose were the most significant EA and carbon source respectively for CA production from lactate by strain CPB6. High titer of CA can be produced from a synthetic substrate containing sucrose, lactate and butyrate. The work provided significant implications for improving CA production in industry-scale.
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WOS关键词	MEDIUM-CHAIN CARBOXYLATES ; UPGRADING DILUTE ETHANOL ; REACTOR MICROBIOMES ; MEGASPHAERA-ELSDENII ; CLOSTRIDIUM-KLUYVERI ; RUMINAL BACTERIUM ; HEXANOIC ACID ; FATTY-ACIDS ; FERMENTATION ; LACTATE
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	BMC
WOS记录号	WOS:000436798400001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30692]
专题	环境治理与食品安全领域_应用与环境微生物研究
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, Environm Microbiol Key Lab Sichuan Prov, Chengdu 610041, Sichuan, Peoples R China; 2.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Auburn Univ, Dept Biosyst Engn, Auburn, AL 36849 USA
推荐引用方式 GB/T 7714	Wang, Han,Li, Xiangzhen,Wang, Yi,et al. Improvement of n-caproic acid production with Ruminococcaceae bacterium CPB6: selection of electron acceptors and carbon sources and optimization of the culture medium[J]. MICROBIAL CELL FACTORIES,2018,17:99.
APA	Wang, Han.,Li, Xiangzhen.,Wang, Yi.,Tao, Yong.,Lu, Shaowen.,...&Li, Daping.(2018).Improvement of n-caproic acid production with Ruminococcaceae bacterium CPB6: selection of electron acceptors and carbon sources and optimization of the culture medium.MICROBIAL CELL FACTORIES,17,99.
MLA	Wang, Han,et al."Improvement of n-caproic acid production with Ruminococcaceae bacterium CPB6: selection of electron acceptors and carbon sources and optimization of the culture medium".MICROBIAL CELL FACTORIES 17(2018):99.