Instability diagnosis and syntrophic acetate oxidation during thermophilic digestion of vegetable waste

doi:10.1016/j.watres.2018.04.019

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 应用与环境微生物研究

	Instability diagnosis and syntrophic acetate oxidation during thermophilic digestion of vegetable waste
	Li, Dong 3; Ran, Yi 1; Chen, Lin 2; Cao, Qin 3; Li, Zhidong 3; Liu, Xiaofeng 3
刊名	WATER RESEARCH
	2018
卷号	139 期号:2018 页码:263-271
关键词	Vegetable waste Thermophilic digestion Process monitoring Instability diagnosis Syntrophic acetate oxidization
ISSN号	0043-1354
DOI	10.1016/j.watres.2018.04.019
产权排序	1
文献子类	Article
英文摘要	Effective process monitoring and instability diagnosis are important for stable anaerobic digestion (AD) of vegetable waste (VW). In order to evaluate the performance of thermophilic digestion of VW, to make early diagnosis for instability after organic overload, and to reveal the dynamics of microbial community under different running states, thermophilic AD of VW was carried out under improved organic loading rates (OLR) of 0.5-2.5 g volatile solid (VS)/(L . d) in this study. Gaseous parameters including volumetric methane production rate (VMPR), CH4, CO2, and H-2 concentrations, and liquid parameters including pH, oxidation reduction potential, volatile fatty acid (VFA), and total alkalinity (TA), bicarbonate alkalinity (BA), intermediate alkalinity (IA), and ammonia, were monitored. The coupling parameters, such as the CH4/CO2, VFA/BA, and BA/TA ratios were also used to evaluate stability. The dynamics of syntrophic acetate-oxidizing bacteria (SAOB), acetoclastic methanogens (AM), and hydrogenotrophic methanogens (HM) were analyzed by high-throughput sequencing. The main methanogenic bacteria were HM (Methanothermobacter) during the start-up period of OLR 0.5 gVS/(L . d), while they were AM (Methanosarcina) during the stable period of OLR of 1.0 gVS/(L . d). The VMPR of stable period was about 0.29 L/(L . d) with total VFA concentration below 100 mg/L, CH4/CO2 > 1.3, and BA/TA>0.9. The first instability due to the accumulation of VFA and self-recovery due to syntrophic acetate oxidation occurred at an OLR of 1.5 g VS/(L . d). The syntrophic acetate-oxidizing bacteria probably belong to genus S1 (family Thermotogaceae). The digestion failed at an OLR of 2.0 g VS/(L . d). H-2 was only detected during collapsed period instead of instable period. The total ammonia nitrogen loss and bicarbonate alkalinity (BA) reduction were the primary causes for the instability of AD of VW without effluent recirculation. Compared with single parameters, the CH4/CO2 and BA/total alkalinity (TA) ratios are recommended as early warning indicators for engineering applications of thermophilic AD of VW. (C) 2018 Elsevier Ltd. All rights reserved.
学科主题	Environment ; Ecology
URL标识	查看原文
WOS关键词	ANAEROBIC CO-DIGESTION ; SP NOV. ; GEN. NOV. ; OXIDIZING BACTERIUM ; FRUIT ; PERFORMANCE ; MANURE ; RECIRCULATION ; FERMENTATION ; ARCHAEON
WOS研究方向	Engineering ; Environmental Sciences & Ecology ; Water Resources
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000434747700026
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30616]
专题	环境治理与食品安全领域_应用与环境微生物研究
作者单位	1.Minist Agr, Biogas Inst, Chengdu 610041, Sichuan, Peoples R China; 2.Changzhou Univ, Inst Urban & Rural Mines, Changzhou 213164, Peoples R China 3.Chinese Acad Sci, Chengdu Inst Biol, Environm Microbiol Key Lab Sichuan Prov, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Li, Dong,Ran, Yi,Chen, Lin,et al. Instability diagnosis and syntrophic acetate oxidation during thermophilic digestion of vegetable waste[J]. WATER RESEARCH,2018,139(2018):263-271.
APA	Li, Dong,Ran, Yi,Chen, Lin,Cao, Qin,Li, Zhidong,&Liu, Xiaofeng.(2018).Instability diagnosis and syntrophic acetate oxidation during thermophilic digestion of vegetable waste.WATER RESEARCH,139(2018),263-271.
MLA	Li, Dong,et al."Instability diagnosis and syntrophic acetate oxidation during thermophilic digestion of vegetable waste".WATER RESEARCH 139.2018(2018):263-271.