Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions

	Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions
	Yao, Chang-Hong 1,2; Ai, Jiang-Ning 1; Cao, Xu-Peng 1; Xue, Song 1
刊名	applied microbiology and biotechnology
	2013-07-01
卷号	97 期号:13 页码:6099-6110
关键词	Tetraselmis subcordiformis Starch Phosphorus Initial cell density Productivity Recovery
通讯作者	薛松
产权排序	1,1
英文摘要	microalgal starch is a potential feedstock for bio-fuel production. nutrient stress is widely used to stimulate starch accumulation in microalgae. cell growth and starch accumulation in the marine green microalga tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell densities (icd) of 1.5, 3.0, 6.0, and 9.0x10(6) cells ml(-1). the intracellular stored phosphorus supported cell growth when extracellular phosphorus was absent. the maximum starch content of 44.1 % was achieved in the lowest icd culture, while the maximum biomass productivity of 0.71 g l-1 day(-1), starch concentration of 1.6 g l-1, and starch productivity of 0.30 g l-1 day(-1) were all obtained in the culture with the icd of 3.0x 10(6) cells ml(-1). appropriate icd could be used to regulate the intracellular phosphorus concentration and maintain adequate photosynthetic activity to achieve the highest starch productivity, along with biomass and starch concentration. the recovery of phosphorus-deprived t. subcordiformis in medium containing 0.5, 1.0, or 6.0 mm kh2po4 was also tested. cell growth and starch accumulation ability could be recovered completely. a phosphorus pool in t. subcordiformis was shown to manipulate its metabolic activity under different environmental phosphorus availability. though lower starch productivity and starch content were achieved under phosphorus deprivation compared with nitrogen-or sulfur-deprived conditions, the higher biomass and starch concentration make t. subcordiformis a good candidate for biomass and starch production under extracellular phosphorus deprivation.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	arthrospira spirulina platensis ; chlamydomonas-reinhardtii ; nutrient limitation ; biomass composition ; carbon allocation ; oil accumulation ; light-intensity ; phosphate ; stress ; photosynthesis
收录类别	SCI
语种	英语
WOS记录号	WOS:000321121500039
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117611]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yao, Chang-Hong,Ai, Jiang-Ning,Cao, Xu-Peng,et al. Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions[J]. applied microbiology and biotechnology,2013,97(13):6099-6110.
APA	Yao, Chang-Hong,Ai, Jiang-Ning,Cao, Xu-Peng,&Xue, Song.(2013).Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions.applied microbiology and biotechnology,97(13),6099-6110.
MLA	Yao, Chang-Hong,et al."Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions".applied microbiology and biotechnology 97.13(2013):6099-6110.