Determination of chitosan content with Schiff base method and HPLC

doi:10.1016/j.ijbiomac.2021.05.121

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Determination of chitosan content with Schiff base method and HPLC
	Miao, Qin 1,2,3; Mi, Yingqi 1,2,3; Cui, Jingmin 1,2,3; Zhang, Jingjing 1,2; Tan, Wenqiang 1,2; Li, Qing 1,2; Guo, Zhanyong 1,2,3
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2021-07-01
卷号	182 页码:1537-1542
关键词	Determination of chitosan content Chitosan-Schiff base HPLC
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2021.05.121
通讯作者	Guo, Zhanyong(zhanyongguo@hotmail.com)
英文摘要	Tremendous awareness of determination of chitosan content accurately is increasing, due to it has great significance to the quality control of chitosan. In this article, two kinds of chitosan-Schiff base derivatives (BCSB and PCSB) were synthesized by the different average degrees of deacetylation (DD) of chitosan with benzaldehyde or propanal, respectively. The total mass of Schiff base derivative product was dried and obtained without washing and loss. Then, a certain amount of the prepared Schiff base compound was taken to hydrolyze into glucosamine hydrochloride (GAH) in strong hydrochloric acidic environment, whose concentration was quantified by HPLC, and the mass of GAH contained in hydrolysis solution could be calculated. Subsequently, the total quality of GAH obtained by hydrolysis of all of the Schiff base product was calculated and obtained, and then the theoretical mass of chitosan could be deduced and calculated by further converse calculation. Finally, the chitosan content was obtained by combining the sample mass used in Schiff base reaction and the theoretical mass of chitosan. This method was accurate and convenient, providing a preeminent idea and method for the determination of chitosan content. (c) 2021 Elsevier B.V. All rights reserved.
资助项目	National Key Research and Development Program of China[2019YFD0900705] ; Natural Science Foundation of Shandong Province of China[ZR2019BD064]
WOS关键词	LIQUID-CHROMATOGRAPHIC DETERMINATION ; ACID-HYDROLYSIS ; ANTIMICROBIAL ACTIVITY ; GLUCOSAMINE ; CHITIN ; DEACETYLATION ; STABILITY ; SAMPLES ; CRAB
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
WOS记录号	WOS:000669494700003
资助机构	National Key Research and Development Program of China ; Natural Science Foundation of Shandong Province of China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29530]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室海岸带生物资源高效利用研究与发展中心
通讯作者	Guo, Zhanyong
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Miao, Qin,Mi, Yingqi,Cui, Jingmin,et al. Determination of chitosan content with Schiff base method and HPLC[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,182:1537-1542.
APA	Miao, Qin.,Mi, Yingqi.,Cui, Jingmin.,Zhang, Jingjing.,Tan, Wenqiang.,...&Guo, Zhanyong.(2021).Determination of chitosan content with Schiff base method and HPLC.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,182,1537-1542.
MLA	Miao, Qin,et al."Determination of chitosan content with Schiff base method and HPLC".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 182(2021):1537-1542.