Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli

doi:10.1007/s12010-020-03453-0

	Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli
	Zhang, Jun 2,3; Tian, Miao 2,3; Chen, Xiaoyan 2,3; Lv, Pengmei 3; Luo, Wen 3; Wang, Zhiyuan 3; Xu, Jingliang 1,3; Wang, Zhongming 3
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2020-11-12
页码	13
关键词	Lipase Lipase chaperone Co-expression Escherichia coli Biodiesel
ISSN号	0273-2289
DOI	10.1007/s12010-020-03453-0
通讯作者	Lv, Pengmei(lvpm@ms.giec.ac.cn) ; Xu, Jingliang(xujl@zzu.edu.cn)
英文摘要	Biodiesel biosynthesis with enzymatic transesterification is considered green, sustainable, and environmentally friendly method. Lipase from Burkholderia cepacia G63 has excellent catalytic properties in biodiesel production. Lipase chaperones promote secretion and folding of enzymes, thereby enhancing enzymatic activity. In the current study, heterologous co-expression of lipase (lipA) and chaperone (lipB) was achieved in Escherichia coli through codon optimization. The enzymatic activity of purified and renatured lipAB was 2080.23 +/- 19.18 U/g at 50 degrees C and pH 8.0. Moreover, lipAB showed increased resistance to pH and temperature changes, and lipAB retained stable catalytic properties after treatment with metal ions, organic solvents, and surfactants, namely Mg2+, methanol, and Triton-100X. Besides, using recombinant lipase lipAB as catalysts, biodiesel was synthesized using rapeseed oil under 50 degrees C for 72 h with a yield of 90.23%. Thus, the current study confirmed that co-expression of lipase and its chaperone is an effective strategy to enhance enzyme activity and improve the biochemical profile, meanwhile, showing that lipAB is a promising biocatalyst for biodiesel production.
资助项目	Guangdong Special Support Program[2017TX04Z109] ; National Natural Science Foundation of China[51606201] ; Science and Technology Planning Project of Guangdong Province[2016A010104008] ; Natural Science Foundation of Guangdong Province[2017A010104010] ; National Key Research and Development Program of China[2019YFB1504003] ; Projects of International Cooperation and Exchanges NSFC[51861145103]
WOS关键词	CODON OPTIMIZATION ; EXPRESSION ; CLONING ; GENES ; OIL
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000589037100001
资助机构	Guangdong Special Support Program ; National Natural Science Foundation of China ; Science and Technology Planning Project of Guangdong Province ; Natural Science Foundation of Guangdong Province ; National Key Research and Development Program of China ; Projects of International Cooperation and Exchanges NSFC
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/34871]
专题	中国科学院广州能源研究所
通讯作者	Lv, Pengmei; Xu, Jingliang
作者单位	1.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China 2.Univ China Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jun,Tian, Miao,Chen, Xiaoyan,et al. Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2020:13.
APA	Zhang, Jun.,Tian, Miao.,Chen, Xiaoyan.,Lv, Pengmei.,Luo, Wen.,...&Wang, Zhongming.(2020).Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,13.
MLA	Zhang, Jun,et al."Co-Expression of a Thermally Stable and Methanol-Resistant Lipase and Its Chaperone from Burkholderia cepacia G63 in Escherichia coli".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2020):13.