The Diversity, Composition, and Putative Functions of Gill-Associated Bacteria of Bathymodiolin Mussel and Vesicomyid Clam from Haima Cold Seep, South China Sea

doi:10.3390/microorganisms8111699

	The Diversity, Composition, and Putative Functions of Gill-Associated Bacteria of Bathymodiolin Mussel and Vesicomyid Clam from Haima Cold Seep, South China Sea
	Ling, Juan 3,4; Guan, Hongxiang 2; Liu, Lihua 2; Tao, Jun 1; Li, Jie 3,4; Dong, Junde 3,4; Zhang, Si 3,4
刊名	MICROORGANISMS
	2020-11-01
卷号	8 期号:11 页码:15
关键词	Haima cold seep mussel and clam gill-associated microbial community potential function
DOI	10.3390/microorganisms8111699
通讯作者	Guan, Hongxiang(guanhx@ms.giec.ac.cn)
英文摘要	The Haima cold seep, which is one of the two active cold seeps in the South China Sea, is known for its great ecological importance. The seep bivalves are assumed to depend mainly on their bacterial symbiosis for survival and growth. However, information on the bacterial diversity, composition, and putative function of gill-associated of dominant dwelling animals in Haima cold seep remain elusive. Herein, we adopted a high-throughput sequencing of 16S rRNA gene amplicons, and function prediction methods (Functional Annotation of Prokaryotic Taxa (FAPROTAX) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICURUSTs)) to purposely illustrate the taxonomic and phylogenetic diversity, composition, and putative functions of the symbionts in bathymodiolin mussel Gigantidas haimaensis (Bivalvia: Mytilidae: Gigantidas) and vesicomyid clam Archivesica marissinica (Bivalvia: Glossoidea: Vesicomyidae). The predominant microbes of both species were Proteobacteria and Gammaproteobacteria on the phylum and class level, respectively. The taxonomic and phylogenetic diversity of gill microbial communities in G. haimaensis were significantly different from those in A. marissinica (p < 0.05). Nine functional groups, including seven carbon-related biogeochemical groups, were identified through the FAPROTAX analysis. However, the most dominant groups for G. haimaensis and A. marissinica were both chemoheterotrophic. G. haimaensis and A. marissinica shared many pathways, however, 16 obtained Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologous groups (42.11%) significantly differed between the two species (p < 0.05). These findings would provide insight into the functions of microbes in the element cycling and energy flow as well as the host-symbiont relationship of bivalves in the Haima cold seep environment.
资助项目	National Key R&D Program of China[2018YFC0310005] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0210] ; NSF of China[91958105] ; Major Program of Guangdong Basic and Applied Research[2019B030302004] ; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[GML2019ZD0401] ; Science and Technology Planning Project of Guangdong Province, China[2017B030314052]
WOS关键词	AUTHIGENIC CARBONATES ; MICROBIAL COMMUNITIES ; DUAL SYMBIOSIS ; GENE ; GREENGENES ; MYTILIDAE ; OXIDATION ; AZORICUS ; BIVALVIA ; TAXONOMY
WOS研究方向	Microbiology
语种	英语
出版者	MDPI
WOS记录号	WOS:000593215400001
资助机构	National Key R&D Program of China ; Qingdao National Laboratory for Marine Science and Technology ; NSF of China ; Major Program of Guangdong Basic and Applied Research ; Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) ; Science and Technology Planning Project of Guangdong Province, China
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/34831]
专题	中国科学院广州能源研究所
通讯作者	Guan, Hongxiang
作者单位	1.Guangzhou Marine Geol Survey, MLR Key Lab Marine Mineral Resources, Guangzhou 510075, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Gas Hydrate, Guangzhou 510640, Peoples R China 3.Southern Marine Sci & Engn Guangdong Lab, Guangzhou 511458, Peoples R China 4.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Peoples R China
推荐引用方式 GB/T 7714	Ling, Juan,Guan, Hongxiang,Liu, Lihua,et al. The Diversity, Composition, and Putative Functions of Gill-Associated Bacteria of Bathymodiolin Mussel and Vesicomyid Clam from Haima Cold Seep, South China Sea[J]. MICROORGANISMS,2020,8(11):15.
APA	Ling, Juan.,Guan, Hongxiang.,Liu, Lihua.,Tao, Jun.,Li, Jie.,...&Zhang, Si.(2020).The Diversity, Composition, and Putative Functions of Gill-Associated Bacteria of Bathymodiolin Mussel and Vesicomyid Clam from Haima Cold Seep, South China Sea.MICROORGANISMS,8(11),15.
MLA	Ling, Juan,et al."The Diversity, Composition, and Putative Functions of Gill-Associated Bacteria of Bathymodiolin Mussel and Vesicomyid Clam from Haima Cold Seep, South China Sea".MICROORGANISMS 8.11(2020):15.