Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production

doi:10.1016/j.fuel.2021.121594

	Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production
	Zhang, Jun 1,3,4; Chen, Xiaoyan 1,4; Lv, Pengmei 4; Luo, Wen 4; Wang, Zhiyuan 4; Xu, Jingliang 2,4; Wang, Zhongming 4
刊名	FUEL
	2021-11-15
卷号	304 页码:11
关键词	Lipase Bionic-immobilization Biodiesel Bio-silicification Molecular dynamics simulation
ISSN号	0016-2361
DOI	10.1016/j.fuel.2021.121594
通讯作者	Lv, Pengmei(lvpm@ms.giec.ac.cn)
英文摘要	In this study, bio-silicification was employed to develop a green and simple bionic-immobilization method for the immobilization of recombinant lipase. The results showed that under the optimized bionic-immobilization conditions, a maximum immobilization efficiency and an activity recovery of 97.71% and 89.62%, respectively, were obtained. Further, bionic-immobilization significantly improved the thermal stability, storage stability, and short-chain alcohol resistance of the bionic-immobilized lipase. Furthermore, molecular dynamics simulation of the bionic-immobilization mechanism revealed that the key amino acid residues that facilitated the binding of the recombinant lipase to silica particles included Arg336, Lys345, His391, His392, His394, and His395, indicating that hydrogen bonds had a special effect in intermolecular recognition, and the active center of the bionic-immobilized lipase favored substrate entry due to the conformational changes of Phe291 and Ile320. Finally, biodiesel was prepared using the bionic-immobilized lipase as catalyst, and after optimizing the enzymatic process, the biodiesel yield reached 91.04% using 20% immobilized lipase dosage within 12 h at 45 degrees C, and a catalytic activity of 55.02% was observed following the reutilization of the bionic-immobilized lipase seven times. These findings suggest that bionic-immobilization is a novel and rapid method to realize recombinant enzyme immobilization, and can provide economic and ecological support for the biocatalytic preparation of industrial products.
资助项目	National Key Research and Development Program of China[2019YFB1504003] ; Projects of International Cooperation and Exchanges NSFC[51861145103] ; Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Program of CAS Key Laboratory of Renewable Energy[E029010801] ; Guangdong Special Support Program[2017TX04Z109] ; National Natural Science Foundation of China[51606201]
WOS关键词	MOLECULAR-DYNAMICS SIMULATION ; ENZYME IMMOBILIZATION ; PROTEIN ; ADSORPTION ; MORPHOLOGY ; SUPPORT
WOS研究方向	Energy & Fuels ; Engineering
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000691213500004
资助机构	National Key Research and Development Program of China ; Projects of International Cooperation and Exchanges NSFC ; Natural Science Foundation of Guangdong Province of China ; Program of CAS Key Laboratory of Renewable Energy ; Guangdong Special Support Program ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/33821]
专题	中国科学院广州能源研究所
通讯作者	Lv, Pengmei
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China 3.Guangdong Ocean Univ, Coll Food Sci & Technol,Guangdong Prov Engn Lab M, Guangdong Prov Engn Technol Res Ctr Seafood, Key Lab Adv Proc Aquat Prod,Guangdong Higher Educ, Zhanjiang 524088, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jun,Chen, Xiaoyan,Lv, Pengmei,et al. Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production[J]. FUEL,2021,304:11.
APA	Zhang, Jun.,Chen, Xiaoyan.,Lv, Pengmei.,Luo, Wen.,Wang, Zhiyuan.,...&Wang, Zhongming.(2021).Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production.FUEL,304,11.
MLA	Zhang, Jun,et al."Bionic-immobilized recombinant lipase obtained via bio-silicification and its catalytic performance in biodiesel production".FUEL 304(2021):11.