Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method

	Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method
	Zhao, Cheng 1,3,5; Yao, Qing-Song 3,7; Wang, Can 2,3,4,6; Luo, Mu-Tan 3,5; Huang, Chao 2,3,4,6; Chen, Xue-Fang 2,3,4,6; Tian, Lan-Lan 3,5; Huang, Qian-Lin 3,5; Xiong, Lian 2,3,4,6; Li, Hai-Long 2,3,4,6
刊名	JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN
	2021-02-01
卷号	43 期号:1 页码:80-87
关键词	Microbial lipid Fluorescent method Lipid content Trichosporon dermatis Lignocellulosic hydrolysates
ISSN号	0253-5106
通讯作者	Huang, Chao(huangchao@ms.giec.ac.cn) ; Chen, Xin-De(cxd_cxd@hotmail.com)
英文摘要	To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R-2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates.
资助项目	National Natural Science Foundation of China[21606229] ; project of Pearl River S&T Nova Program of Guangzhou[201610010014] ; Science and Technology Project of Guangdong Province[2016A010105016] ; Foundation Project of Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development[y809jj1001] ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences[y407r41001]
WOS关键词	NILE RED ; INTRACELLULAR LIPIDS ; OIL PRODUCTION ; MICROALGAE
WOS研究方向	Chemistry
语种	英语
出版者	CHEM SOC PAKISTAN
WOS记录号	WOS:000647273100010
资助机构	National Natural Science Foundation of China ; project of Pearl River S&T Nova Program of Guangzhou ; Science and Technology Project of Guangdong Province ; Foundation Project of Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development ; Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/33101]
专题	中国科学院广州能源研究所
通讯作者	Huang, Chao; Chen, Xin-De
作者单位	1.South China Inst Environm Sci, Guangdong Key Lab Water & Air Pollut Control, Minist Ecol & Environm China, Guangzhou 510535, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Chinese Acad Sci, Guangzhou Inst Energy Convers, R&D Ctr Xuyi Attapulgite Appl Technol, Xuyi 211700, Peoples R China 7.Univ Sci & Technol China, Nano Sci & Technol Inst, Suzhou 215123, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Cheng,Yao, Qing-Song,Wang, Can,et al. Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method[J]. JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN,2021,43(1):80-87.
APA	Zhao, Cheng.,Yao, Qing-Song.,Wang, Can.,Luo, Mu-Tan.,Huang, Chao.,...&Chen, Xin-De.(2021).Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method.JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN,43(1),80-87.
MLA	Zhao, Cheng,et al."Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method".JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN 43.1(2021):80-87.