Study of lncRNA TPA in Promoting Invasion and Metastasis of Breast Cancer Mediated by TGF-beta Signaling Pathway

doi:10.3389/fcel.2021.688751

	Study of lncRNA TPA in Promoting Invasion and Metastasis of Breast Cancer Mediated by TGF-beta Signaling Pathway
	Li, Qinglin; Mo, Wenju; Ding, Yuqin; Ding, Xiaowen
刊名	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
	2021-08-06
卷号	9
关键词	prognostic significance lncRNA TPA TGF-beta metastasis breast cancer
ISSN号	2296-634X
DOI	10.3389/fcel.2021.688751
通讯作者	Ding, Xiaowen(xiaowding@163.com)
英文摘要	Purpose: This study was to investigate the effects of lncRNA TPA overexpression and knockdown in stable transfected cell lines on the EMT, migration and invasion capabilities of breast cancer cells. Methods: WB and qRT-PCR were used to detect the expression of E-cadherin, Vimentin, fibronectin and N-cadherin, the key molecules of EMT, to determine whether lncRNA regulates EMT; scratch, migration and invasion assay were used to detected the effect of lncRNA TPA on the migration and invasion of breast cancer cells. The effect of lncRNA TPA on breast cancer metastasis was observed in nude mice model. Pierce Magnetic RNA-Protein Pull-Down Kit was used to bind the 3'-terminal desulfurized biotin-labeled lncRNA TPA with Magnetic beads, and then incubated with the proteins extracted from cell line C and D, respectively. After elution of the binding proteins, the interacting proteins were further identified by mass spectrometry to screen out the interacting proteins. The candidate proteins were expressed and purified in vitro, and the interaction between lncRNA-candidate proteins were verified by RNA-EMSA. Results: Overexpression of lncRNA TPA decreased the expression of E-cadherin, and significantly increased the expression of Vimentin, fibronectin and TGF-beta (p < 0.01), and increased the migration rate, migration ability and invasion ability of cell group (P < 0.01). Multiple lung metastases were observed in the lung tissue of nude mice with overexpression of lncRNA TPA. Conclusion: LncRNA TPA affects the occurrence of breast cancer EMT through TGF-beta signaling pathway, and then promotes the invasion and metastasis of breast cancer. LncRNA TPA may affect the corresponding signaling pathways through one or more interacting proteins, and ultimately promote the invasion and metastasis of breast cancer.
资助项目	Natural Science Foundation of Zhejiang Province[LY18H160034] ; Zhejiang Province Traditional Chinese Medicine Scientific Research Fund Project[2016ZQ009] ; Zhejiang Province Traditional Chinese Medicine Scientific Research Fund Project[2020ZQ005] ; Zhejiang Medical and Health Science and Technology Project[2016KYB038] ; Zhejiang Medical and Health Science and Technology Project[2019ZD024]
WOS关键词	LONG NONCODING RNA ; EPITHELIAL-MESENCHYMAL TRANSITION ; E-CADHERIN ; EMT ; SNAIL ; PROLIFERATION ; STATISTICS ; HOTAIR ; MALAT1 ; GROWTH
WOS研究方向	Cell Biology ; Developmental Biology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000684983400002
资助机构	Natural Science Foundation of Zhejiang Province ; Zhejiang Province Traditional Chinese Medicine Scientific Research Fund Project ; Zhejiang Medical and Health Science and Technology Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/125153]
专题	中国科学院合肥物质科学研究院
通讯作者	Ding, Xiaowen
作者单位	Chinese Acad Sci, Univ Chinese Acad Sci, Zhejiang Canc Hosp, Inst Basic Med & Canc IBMC,Canc Hosp, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Li, Qinglin,Mo, Wenju,Ding, Yuqin,et al. Study of lncRNA TPA in Promoting Invasion and Metastasis of Breast Cancer Mediated by TGF-beta Signaling Pathway[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2021,9.
APA	Li, Qinglin,Mo, Wenju,Ding, Yuqin,&Ding, Xiaowen.(2021).Study of lncRNA TPA in Promoting Invasion and Metastasis of Breast Cancer Mediated by TGF-beta Signaling Pathway.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,9.
MLA	Li, Qinglin,et al."Study of lncRNA TPA in Promoting Invasion and Metastasis of Breast Cancer Mediated by TGF-beta Signaling Pathway".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9(2021).