The Impact of Host Genotype, Intestinal Sites and Probiotics Supplementation on the Gut Microbiota Composition and Diversity in Sheep

doi:10.3390/biology10080769

	The Impact of Host Genotype, Intestinal Sites and Probiotics Supplementation on the Gut Microbiota Composition and Diversity in Sheep
	Wang, Xiaoqi 1,2,3; Zhang, Zhichao 3; Wang, Xiaoping 3; Bao, Qi 3; Wang, Rujing 1,2; Duan, Ziyuan 3
刊名	BIOLOGY-BASEL
	2021-08-01
卷号	10
关键词	gut microbiota composition intestinal sites sheep breeds Bifidobacterium feces
DOI	10.3390/biology10080769
通讯作者	Wang, Rujing(rjwang@iim.ac.cn) ; Duan, Ziyuan(zyduan@genetics.ac.cn)
英文摘要	Simple Summary Given the fact that sheep serve as an increased meat product consumption in China, as well as a suitable model for fat deposition, their digestive tract microbiota has drawn growing attention. Our study depicted the gut microbiota community composition and diversity of sheep with varied genotypes but shared geography, with different sampling intestinal sites and probiotics supplementation time. The results indicated the great difference of the gut microbiota in samples from different sheep breeds, various intestinal sites, and different probiotics feeding times. Additionally, all results implied the dominance of the host factor in shaping unique microbiota under a certain environment, the greatest similarity of colonic and fecal microbiota, and the oral probiotic effectiveness for a given period of time for sheep. Three sampling strategies with a 16s rRNA high-throughput sequencing and gene expression assay (by RT-PCR) were designed, to better understand the host and probiotics effect on gut microbiota in sheep. Sampling: (1) colon contents and back-fat tissues from small-tailed Han sheep (SHS), big-tailed Hulun Buir sheep (BHBS), and short-tailed Steppe sheep (SHBS) (n = 12, 14, 12); (2) jejunum, cecum and colon contents, and feces from Tan sheep (TS, n = 6); (3) feces from TS at 4 time points (nonfeeding, 30 and 60 feeding days, and stop feeding 30 days) with probiotics supplementation (n = 7). The results indicated SHS had the highest Firmicutes abundance, the thinnest back-fat, and the lowest expression of C/EBP beta, C/EBP delta, ATGL, CFD, and SREBP1. Some bacteria orders and families could be potential biomarkers for sheep breeds with a distinct distribution of bacterial abundance, implying the host genotype is predominant in shaping unique microbiota under a shared environment. The microbiota diversity and Bifidobacterial populations significantly changed after 60 days of feeding but restored to its initial state, with mostly colonies, after 30 days ceased. The microbiota composition was greatly different between the small and large intestines, but somewhat different between the large intestine and feces; feces may be reliable for studying large intestinal microbiota in ruminants.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA 26040305] ; National Key R&D Program of China[2016YFC0500709] ; Key Projects of Chinese Academy of Sciences[KFZD-SW-219]
WOS关键词	MODULATE ; DATABASE ; COLON ; FECES ; CECUM
WOS研究方向	Life Sciences & Biomedicine - Other Topics
语种	英语
出版者	MDPI
WOS记录号	WOS:000688923000001
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key R&D Program of China ; Key Projects of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/125149]
专题	中国科学院合肥物质科学研究院
通讯作者	Wang, Rujing; Duan, Ziyuan
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaoqi,Zhang, Zhichao,Wang, Xiaoping,et al. The Impact of Host Genotype, Intestinal Sites and Probiotics Supplementation on the Gut Microbiota Composition and Diversity in Sheep[J]. BIOLOGY-BASEL,2021,10.
APA	Wang, Xiaoqi,Zhang, Zhichao,Wang, Xiaoping,Bao, Qi,Wang, Rujing,&Duan, Ziyuan.(2021).The Impact of Host Genotype, Intestinal Sites and Probiotics Supplementation on the Gut Microbiota Composition and Diversity in Sheep.BIOLOGY-BASEL,10.
MLA	Wang, Xiaoqi,et al."The Impact of Host Genotype, Intestinal Sites and Probiotics Supplementation on the Gut Microbiota Composition and Diversity in Sheep".BIOLOGY-BASEL 10(2021).