Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme

doi:10.1021/acscatal.1c00287

	Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme
	Kang, Fuying 1,2; Yu, Lu 3; Xia, Yan 1,2; Yu, Minling 1,2; Xia, Lin 4; Wang, Yuchuan 4; Yang, Lin 5,6; Wang, Tianyuan 2; Gong, Weimin 5,6; Tian, Changlin 3,5,6
刊名	ACS CATALYSIS
	2021-05-07
卷号	11
关键词	photosystem I rational design photosensitizer protein iron-sulfur (Fe4S4) cluster photocatalytic CO2 reductase
ISSN号	2155-5435
DOI	10.1021/acscatal.1c00287
通讯作者	Tian, Changlin(cltian@ustc.edu.cn) ; Liu, Xiaohong(liuxh@moon.ibp.ac.cn) ; Wang, Jiangyun(jwang@ibp.ac.cn)
英文摘要	Photosystem I (PSI) is a very large membrane protein complex (similar to 1000 kDa) harboring P700, the strongest reductant known in biological systems, which is responsible for driving NAD(P)(+) and ultimately for CO2 reduction. Although PSI is one of the most important components in the photosynthesis machinery, it has remained difficult to enhance PSI functions through genetic engineering due to its enormous complexity. Inspired by PSI's ability to undergo multiple-step photo-induced electron hopping from P700 to iron-sulfur [Fe4S4] clusters, we designed a 33 kDa miniature photocatalytic CO2-reducing enzyme (mPCE) harboring a chromophore (BpC) and two [Fe4S4] clusters (Fe-A/Fe-B). Through reduction potential fine-tuning, we optimized the multiple-step electron hopping from BpC to Fe-A/Fe-B, culminating in a CO2/HCOOH conversion quantum efficiency of 1.43%. As mPCE can be overexpressed with a high yield in Escherichia coli cells without requiring synthetic cofactors, further development along this route may result in rapid photo-enzyme quantum yield improvement and functional expansion through an efficient directed evolution process.
资助项目	National Key Research and Development Program of China[2019YFA0904101] ; National Key Research and Development Program of China[2019YFA0405600] ; National Key Research and Development Program of China[2017YFA0503704] ; National Science Foundation of China[21750003] ; National Science Foundation of China[21890743] ; National Science Foundation of China[21837005] ; National Science Foundation of China[21825703] ; National Science Foundation of China[21927814] ; National Science Foundation of China[U1732275] ; Sanming Project of Medicine in Shenzhen[SZSM201811092] ; Youth Innovation Promotion Association CAS ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB37040000] ; Users with Excellence Program of Hefei Science Center, CAS[2019HSC-UE005]
WOS关键词	VINELANDII FE PROTEIN ; ELECTRON-TRANSFER ; REDUCTION POTENTIALS ; ENERGY-TRANSFER ; PHOTOSYSTEM-I ; REDOX STATE ; IRON ; CLUSTERS ; FERREDOXIN ; SEQUENCE
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000649106200062
资助机构	National Key Research and Development Program of China ; National Science Foundation of China ; Sanming Project of Medicine in Shenzhen ; Youth Innovation Promotion Association CAS ; Strategic Priority Research Program of Chinese Academy of Sciences ; Users with Excellence Program of Hefei Science Center, CAS
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/123666]
专题	中国科学院合肥物质科学研究院
通讯作者	Tian, Changlin; Liu, Xiaohong; Wang, Jiangyun
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Lab RNA Biol, Beijing 100101, Peoples R China 3.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China 4.Chinese Acad Sci, Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China 5.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China 6.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
推荐引用方式 GB/T 7714	Kang, Fuying,Yu, Lu,Xia, Yan,et al. Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme[J]. ACS CATALYSIS,2021,11.
APA	Kang, Fuying.,Yu, Lu.,Xia, Yan.,Yu, Minling.,Xia, Lin.,...&Wang, Jiangyun.(2021).Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme.ACS CATALYSIS,11.
MLA	Kang, Fuying,et al."Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme".ACS CATALYSIS 11(2021).