Clinical Relevance of PD-L1 Expression and CD8+T Cells' Infiltration in Patients With Lung Invasive Mucinous Adenocarcinoma

doi:10.3389/fonc.2021.683432

	Clinical Relevance of PD-L1 Expression and CD8+T Cells' Infiltration in Patients With Lung Invasive Mucinous Adenocarcinoma
	Xu, Xiaoling 1,2,3; Li, Na 1,3; Wang, Ding 1; Chen, Wei 3; Fan, Yun 1,2,3
刊名	FRONTIERS IN ONCOLOGY
	2021-06-24
卷号	11
关键词	invasive mucinous adenocarcinoma lung cancer PD-L1 expression CD8+T cells tumor microenvironment genetic characteristics treatment
ISSN号	2234-943X
DOI	10.3389/fonc.2021.683432
通讯作者	Fan, Yun(fanyun1218@163.com)
英文摘要	Background Invasive mucinous adenocarcinoma (IMA) of the lung is a rare and distinct subtype of adenocarcinoma. At present, people have no idea whether IMA patients can benefit from immunotherapy and target therapy; thus there is an urgent need to clarify the immune microenvironment and genetic characteristics of this cohort. Methods A total of 31 IMA patients matched with 27 non-mucinous adenocarcinoma (non-IMA) patients were enrolled in this study, and clinical data was collected. The expression of PD-L1, CD8+ tumor-infiltrating lymphocytes (TILs) and ALK was determined by immunohistochemistry. Polymerase Chain Reaction was used to determine the mutations of EGFR. The Chi-square test, Kaplan-Meier method and Cox proportional hazard regression model were used to explore the correlations between these clinicopathological variables, survival and identify risk factors. Results Of the patients with IMA 9.7% (3/31) revealed positive PD-L1 expression and 35.5% (11/31) showed CD8+ TIL infiltration, which were markedly lower than that of non-IMA group [PD-L1: 48.1% (13/27); CD8: 81.5% (22/27)]. Moreover, five (16.1%) patients in IMA group and 10 (37.0%) patients in non-IMA group had EGFR mutations, and nine (29.0%) patients in IMA group and zero (0.0%) patient in non-IMA group had ALK rearrangements. Additionally, we observed that IMA patients with CD8+ TIL infiltration had a worse prognosis than CD8-negative group (P = 0.024). Multivariate analyses showed that CD8 was an independent prognostic factor for patient's survival (HR = 5.60, 95% CI: 1.35-23.22, P = 0.017). Conclusion Patients with IMA have down-regulated expression of PD-L1 and less CD8+ TIL infiltration in tumor microenvironment. Besides, a lower frequency of EGFR mutations was detected in patients with IMA than non-IMA patients while a higher rate of ALK rearrangements was found. Our results provide important reference for therapy of lung IMA.
资助项目	National Natural Science Foundation of China[81802995] ; National Natural Science Foundation of China[81672315] ; Zhejiang Province Public Welfare Funds[LGF19H280004]
WOS关键词	CANCER ; MICROENVIRONMENT ; CLASSIFICATION ; SURVIVAL ; BIOLOGY ; FUSIONS ; IMPACT ; KRAS
WOS研究方向	Oncology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000670647200001
资助机构	National Natural Science Foundation of China ; Zhejiang Province Public Welfare Funds
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/123580]
专题	中国科学院合肥物质科学研究院
通讯作者	Fan, Yun
作者单位	1.Zhejiang Chinese Med Univ, Clin Med Coll 2, Dept Med Oncol, Hangzhou, Peoples R China 2.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Key Lab Head & Neck Canc Translat Res Zhejiang Pr, Canc Hosp, Hangzhou, Peoples R China 3.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xiaoling,Li, Na,Wang, Ding,et al. Clinical Relevance of PD-L1 Expression and CD8+T Cells' Infiltration in Patients With Lung Invasive Mucinous Adenocarcinoma[J]. FRONTIERS IN ONCOLOGY,2021,11.
APA	Xu, Xiaoling,Li, Na,Wang, Ding,Chen, Wei,&Fan, Yun.(2021).Clinical Relevance of PD-L1 Expression and CD8+T Cells' Infiltration in Patients With Lung Invasive Mucinous Adenocarcinoma.FRONTIERS IN ONCOLOGY,11.
MLA	Xu, Xiaoling,et al."Clinical Relevance of PD-L1 Expression and CD8+T Cells' Infiltration in Patients With Lung Invasive Mucinous Adenocarcinoma".FRONTIERS IN ONCOLOGY 11(2021).