Down-regulation of BCL2L13 renders poor prognosis in clear cell and papillary renal cell carcinoma

doi:10.1186/s12935-021-02039-y

	Down-regulation of BCL2L13 renders poor prognosis in clear cell and papillary renal cell carcinoma
	Meng, Fei 1,2,6; Zhang, Luojin 3; Zhang, Mingjun 4; Ye, Kaiqin 1,2; Guo, Wei 1,6; Liu, Yu 1,2,6; Yang, Wulin 1,2; Zhai, Zhimin 3; Wang, Hongzhi 1,2; Xiao, Jun 5
刊名	CANCER CELL INTERNATIONAL
	2021-06-30
卷号	21
关键词	BCL-rambo Cell death Renal cancer Prognosis ANT
DOI	10.1186/s12935-021-02039-y
通讯作者	Xiao, Jun(ixiaojun@ustc.edu.cn) ; Dai, Haiming(daih@cmpt.ac.cn)
英文摘要	BackgroundBCL2L13 belongs to the BCL2 super family, with its protein product exhibits capacity of apoptosis-mediating in diversified cell lines. Previous studies have shown that BCL2L13 has functional consequence in several tumor types, including ALL and GBM, however, its function in kidney cancer remains as yet unclearly.MethodsMultiple web-based portals were employed to analyze the effect of BCL2L13 in kidney cancer using the data from TCGA database. Functional enrichment analysis and hubs of BCL2L13 co-expressed genes in clear cell renal cell carcinoma (ccRCC) and papillary renal cell carcinoma (pRCC) were carried out on Cytoscape. Evaluation of BCL2L13 protein level was accomplished through immunohistochemistry on paraffin embedded renal cancer tissue sections. Western blotting and flow cytometry were implemented to further analyze the pro-apoptotic function of BCL2L13 in ccRCC cell line 786-0.ResultsBCL2L13 expression is significantly decreased in ccRCC and pRCC patients, however, mutations and copy number alterations are rarely observed. The poor prognosis of ccRCC that derived from down-regulated BCL2L13 is independent of patients' gender or tumor grade. Furthermore, BCL2L13 only weakly correlates with the genes that mutated in kidney cancer or the genes that associated with inherited kidney cancer predisposing syndrome, while actively correlates with SLC25A4. As a downstream effector of BCL2L13 in its pro-apoptotic pathway, SLC25A4 is found as one of the hub genes that involved in the physiological function of BCL2L13 in kidney cancer tissues.ConclusionsDown-regulation of BCL2L13 renders poor prognosis in ccRCC and pRCC. This disadvantageous factor is independent of any well-known kidney cancer related genes, so BCL2L13 can be used as an effective indicator for prognostic evaluation of renal cell carcinoma.
资助项目	National Natural Science Foundation of China[31970701] ; National Natural Science Foundation of China[21772201] ; Anhui Medical University ; Center of Medical Physics and Technology[LHJJ202006] ; Center of Medical Physics and Technology[LHJJ202007]
WOS关键词	BCL-RAMBO ; APOPTOSIS ; KIDNEY ; CANCER ; GENE ; EXPRESSION ; RESISTANCE
WOS研究方向	Oncology
语种	英语
出版者	BMC
WOS记录号	WOS:000671297500008
资助机构	National Natural Science Foundation of China ; Anhui Medical University ; Center of Medical Physics and Technology
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/123520]
专题	中国科学院合肥物质科学研究院
通讯作者	Xiao, Jun; Dai, Haiming
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Hlth & Med Technol, Anhui Prov Key Lab Med Phys & Technol, 350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China 2.Hefei Canc Hosp, Chinese Acad Sci, Hefei 230031, Peoples R China 3.Anhui Med Univ, Affiliated Hosp 2, Dept Hematol, Hefei 230601, Peoples R China 4.Anhui Med Univ, Affiliated Hosp 2, Dept Oncol, Hefei 230601, Peoples R China 5.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Urol, Hefei 230001, Peoples R China 6.Univ Sci & Technol China, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Meng, Fei,Zhang, Luojin,Zhang, Mingjun,et al. Down-regulation of BCL2L13 renders poor prognosis in clear cell and papillary renal cell carcinoma[J]. CANCER CELL INTERNATIONAL,2021,21.
APA	Meng, Fei.,Zhang, Luojin.,Zhang, Mingjun.,Ye, Kaiqin.,Guo, Wei.,...&Dai, Haiming.(2021).Down-regulation of BCL2L13 renders poor prognosis in clear cell and papillary renal cell carcinoma.CANCER CELL INTERNATIONAL,21.
MLA	Meng, Fei,et al."Down-regulation of BCL2L13 renders poor prognosis in clear cell and papillary renal cell carcinoma".CANCER CELL INTERNATIONAL 21(2021).