Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway

doi:10.1093/bbb/zbab074

	Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway
	Ni, Zhijian 1,4; Wu, Jinyong 2,3,4; Li, Zhongkui 1,4; Yuan, Lixia 4; Wang, Yu 3; Chen, Xiangsong 2,4; Yao, Jianming 1,4
刊名	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
	2021-06-24
卷号	85
关键词	Escherichia coli 3-fucosyllactose cofactors sorbitol fructose-6-phosphate
ISSN号	0916-8451
DOI	10.1093/bbb/zbab074
通讯作者	Chen, Xiangsong(xschen@ipp.ac.cn) ; Yao, Jianming(jmyao@ipp.ac.cn)
英文摘要	3-fucosyllactose (3-FL) and 2'-fucosyllactose (2'-FL), are two important fucosylated oligosaccharides in human milk. Extensive studies on 2'-FL enabled its official approval for use in infant formula. However, development of 3-FL has been somewhat sluggish due to its low content in human milk and poor yield in enlarged production. Here, an alpha-1,3-fucosyltransferase mutant was introduced into an engineered Escherichia coli (E. coli) capable of producing GDP-L-fucose, leading to a promising 3-FL titer in a 5.0-L bioreactor. To increase the availability of cofactors (NADPH and GTP) for optimized 3-FL production, zwf, pntAB, and gsk genes were successively overexpressed, finally resulting in a higher 3-FL level with a titer of 35.72 g/L and a yield of 0.82 mol 3-FL/mol lactose. Unexpectedly, the deletion of pfkA gene led to a much lower performance of 3-FL production than the control strain. Still, our strategy achieved the highest 3-FL level in E. coli to date. [GRAPHICS] The metabolically engineered Escherichia coli effectively produced 3-fucosyllactose through the modified alpha-1,3-fucosyltransferase and the enhanced regeneration of cofactors (NADPH and GTP).
资助项目	Science and Technology Service Network Initiative of Chinese Academy of Science[KFJ-STS-QYZD-122] ; Science and Technology Planning Project of Wuhan City[2020020602012119]
WOS关键词	HUMAN-MILK OLIGOSACCHARIDES ; BIOTECHNOLOGICAL PRODUCTION ; HELICOBACTER-PYLORI ; FUTILE CYCLE ; ALPHA-1,3-FUCOSYL-TRANSFERASE ; 2'-FUCOSYLLACTOSE ; TRANSHYDROGENASE ; PCR
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000671178200026
资助机构	Science and Technology Service Network Initiative of Chinese Academy of Science ; Science and Technology Planning Project of Wuhan City
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/123475]
专题	中国科学院合肥物质科学研究院
通讯作者	Chen, Xiangsong; Yao, Jianming
作者单位	1.Univ Sci & Technol China, Sci Isl Branch Grad Sch, Hefei, Peoples R China 2.Chinese Acad Sci, Huainan New Energy Res Ctr, Inst Plasma Phys, Huainan, Peoples R China 3.Wuhan Zhongke Opt Valley Green Biotechnol Co Ltd, Wuhan, Peoples R China 4.Chinese Acad Sci, Inst Plasma Phys, Hefei Inst Phys Sci, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Ni, Zhijian,Wu, Jinyong,Li, Zhongkui,et al. Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2021,85.
APA	Ni, Zhijian.,Wu, Jinyong.,Li, Zhongkui.,Yuan, Lixia.,Wang, Yu.,...&Yao, Jianming.(2021).Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,85.
MLA	Ni, Zhijian,et al."Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 85(2021).