Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway

doi:10.1155/2021/8100298

	Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway
	Chen, Jianbin 2; Zhang, Can 3; Li, Shouye 4; Li, Zheming 4; Lai, Xiaojing 1,5; Xia, Qingqing 6
刊名	JOURNAL OF IMMUNOLOGY RESEARCH
	2021-07-14
卷号	2021
ISSN号	2314-8861
DOI	10.1155/2021/8100298
通讯作者	Lai, Xiaojing(czz20160127@163.com) ; Xia, Qingqing(418714257@qq.com)
英文摘要	Background. Spinal cord injury (SCI) remains a challenge owing to limited therapies. The exosome of neural stem cells (NSCs-Exos) and FTY720 transplantation could improve SCI effectively. However, the effect and mechanism of NSCs-Exos combined with FTY720 (FTY720-NSCs-Exos) transplantation in the treatment of SCI are not fully understood. Methods. Sprague Dawley rats (8-week-old) were used to establish the SCI model, followed by the treatment of NSCs-Exos, FTY720, and FTY720-NSCs-Exos. The effect of FTY720, NSCs-Exos, and FTY720-NSCs-Exos combination treatment on hindlimb function, pathological changes, apoptosis activity, and the expression of spinal edema-related proteins and apoptosis-related proteins in SCI models were investigated by BBB scoring, HE staining, TUNEL staining and immunohistochemistry, and Western blotting. Meanwhile, the effect of these treatments on spinal cord microvascular endothelial cells (SCMECs) was detected under hypoxic circumstance. Results. Our results found that FTY720-NSCs-Exos could alleviate pathological alterations and ameliorate the hindlimb function and oxygen insufficiency in model mice after SCI. In addition, exosomes could ameliorate the morphology of neurons, reduce inflammatory infiltration and edema, decrease the expression of Bax and AQP-4, upregulate the expression of claudin-5 and Bcl-2, and inhibit cell apoptosis. At the same time, in vitro experiments showed that FTY720-NSCs-Exos could protect the barrier of SCMECs under hypoxic circumstance, and the mechanism is related to PTEN/AKT pathway. Conclusion. FTY720-NSCs-Exos therapy displayed a positive therapeutic effect on SCI by regulating PTEN/AKT pathway and offered a new therapy for SCI.
资助项目	Basic Public Welfare Research Project of Zhejiang Province[LGF19H060001]
WOS关键词	MICROVASCULAR PERICYTES ; BRAIN ; EXPRESSION ; NEURONS ; EDEMA
WOS研究方向	Immunology
语种	英语
出版者	HINDAWI LTD
WOS记录号	WOS:000680840700004
资助机构	Basic Public Welfare Research Project of Zhejiang Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/123212]
专题	中国科学院合肥物质科学研究院
通讯作者	Lai, Xiaojing; Xia, Qingqing
作者单位	1.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Banshan East Rd 1, Hangzhou 310022, Zhejiang, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Neurosurg, Wuhan 430030, Hubei, Peoples R China 3.Hunan Univ, Coll Biol, Dept Biomed Engn, Changsha 410082, Hunan, Peoples R China 4.Hangzhou Med Coll, Coll Pharm, Hangzhou 310022, Zhejiang, Peoples R China 5.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 6.Wenzhou Med Univ, Taizhou Peoples Hosp 1, Huangyan Hosp, Dept Lab Med, Hengjie Rd 218, Taizhou 318020, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Chen, Jianbin,Zhang, Can,Li, Shouye,et al. Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway[J]. JOURNAL OF IMMUNOLOGY RESEARCH,2021,2021.
APA	Chen, Jianbin,Zhang, Can,Li, Shouye,Li, Zheming,Lai, Xiaojing,&Xia, Qingqing.(2021).Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway.JOURNAL OF IMMUNOLOGY RESEARCH,2021.
MLA	Chen, Jianbin,et al."Exosomes Derived from Nerve Stem Cells Loaded with FTY720 Promote the Recovery after Spinal Cord Injury in Rats by PTEN/AKT Signal Pathway".JOURNAL OF IMMUNOLOGY RESEARCH 2021(2021).