Long non-coding RNA MCM3AP antisense RNA 1 promotes non-small cell lung cancer progression through targeting microRNA-195-5p

doi:10.1080/21655979.2021.1950282

	Long non-coding RNA MCM3AP antisense RNA 1 promotes non-small cell lung cancer progression through targeting microRNA-195-5p
	Shen, Dijian 1,2; Li, Jianqiang 1,2; Tao, Kaiyi 1,2; Jiang, Youhua 1,2
刊名	BIOENGINEERED
	2021
卷号	12
关键词	MCM3AP-AS1 miR-195-5p E2F3 NSCLC
ISSN号	2165-5979
DOI	10.1080/21655979.2021.1950282
通讯作者	Jiang, Youhua(jiangyh@zjcc.org.cn)
英文摘要	Lung cancer (LC) ranks first among all causes of cancer-related death, with non-small cell lung cancer (NSCLC) taking up 85% of lung cancer cases. Although lncRNA MCM3AP antisense RNA 1 (MCM3AP-AS1) has been reported to be an oncogenic factor in NSCLC, its detailed mechanism in NSCLC is unknown. In this study, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine MCM3AP-AS1, microRNA (miR)-195-5p and E2F transcription factor 3 (E2F3) mRNA expressions in NSCLC tissues and cells. Western blot was utilized to determine the expression levels of E2F3, BCL2-associated X protein (Bax), B-cell lymphoma-2 (Bcl-2), E-cadherin and N-cadherin. CCK-8 and Transwell assays were conducted to examine cell proliferation, migration and invasion, respectively. Dual-luciferase reporter assay and RNA immunoprecipitation experiments were used to determine the regulatory relationships between MCM3AP-AS1 and miR-195-5p, and miR-195-5p and E2F3. We demonstrated that MCM3AP-AS1 was overexpressed in NSCLC tissues and cells, and MCM3AP-AS1 overexpression accelerated the proliferation, migration and invasion of NSCLC cells. In addition, MCM3AP-AS1 overexpression markedly up-modulated Bcl-2 expression and repressed Bax expression; MCM3AP-AS1 overexpression also significantly upregulated N-cadherin expression and suppressed E-cadherin expression in NSCLC cells. What is more, in NSCLC cells, miR-195-5p was a target of MCM3AP-AS1, and the latter worked as a molecular sponge for miR-195-5p to regulate E2F3 expression. Collectively, MCM3AP-AS1, serving as a competitive endogenous RNA (ceRNA) to regulate miR-195-5p/E2F3 axis, promotes NSCLC progression, which is a promising therapeutic target for NSCLC. [GRAPHICS] .
WOS关键词	INHIBITS PROLIFERATION ; EXPRESSION ; METASTASIS ; MIR-195-5P ; APOPTOSIS ; MIGRATION ; INVASION ; BINDING
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000681234800001
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/123057]
专题	中国科学院合肥物质科学研究院
通讯作者	Jiang, Youhua
作者单位	1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Thorac Surg, Hangzhou, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Canc & Basic Med IBMC, 1 Banshan East Rd, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Shen, Dijian,Li, Jianqiang,Tao, Kaiyi,et al. Long non-coding RNA MCM3AP antisense RNA 1 promotes non-small cell lung cancer progression through targeting microRNA-195-5p[J]. BIOENGINEERED,2021,12.
APA	Shen, Dijian,Li, Jianqiang,Tao, Kaiyi,&Jiang, Youhua.(2021).Long non-coding RNA MCM3AP antisense RNA 1 promotes non-small cell lung cancer progression through targeting microRNA-195-5p.BIOENGINEERED,12.
MLA	Shen, Dijian,et al."Long non-coding RNA MCM3AP antisense RNA 1 promotes non-small cell lung cancer progression through targeting microRNA-195-5p".BIOENGINEERED 12(2021).