A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion

doi:10.1016/j.devcel.2021.08.015

	A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion
	Lyu, Xuchao 12,13; Wang, Jia 12,13; Wang, Jianqin 12,13; Yin, Ye-Sheng 11; Zhu, Yun 10; Li, Lin-Lin 9; Huang, Shuangru 6,7,8; Peng, Shuang 5; Xue, Boxin 10; Liao, Rongyu 12,13
刊名	DEVELOPMENTAL CELL
	2021-09-27
卷号	56 期号:18 页码:2592-+
ISSN号	1534-5807
DOI	10.1016/j.devcel.2021.08.015
通讯作者	Chen, Feng-Jung(derrick_chen@fudan.edu.cn) ; Li, Peng(li-peng@tsinghua.edu.cn)
英文摘要	Membrane contact between intracellular organelles is important in mediating organelle communication. However, the assembly of molecular machinery at membrane contact site and its internal organization correlating with its functional activity remain unclear, Here, we demonstrate that a gel-like condensation of Cidec, a crucial protein for obesity development by facilitating lipid droplet (LD) fusion, occurs at the LD-LD contact site (LDCS) through phase separation. The homomeric interaction between the multivalent N terminus of Cidec is sufficient to promote its phase separation both in vivo and in vitro. Interestingly, Cidec condensation at LDCSs generates highly plastic and lipid-permeable fusion plates that are geometrically constrained by donor LDs. In addition, Cidec condensates are distributed unevenly in the fusion plate generating stochastic sub-compartments that may represent unique lipid passageways during LD fusion. We have thus uncovered the organization and functional significance of geometry-constrained Cidec phase separation in mediating LD fusion and lipid homeostasis.
资助项目	Tsinghua University ; National Key R&D Program of China[2018YFA050 6901] ; National Key R&D Program of China[2018YFA0800301] ; National Key R&D Program of China[2016YFA0502002] ; National Key R&D Program of China[2019YFA08 01701] ; National Natural Science Foundation of China[91857103] ; National Natural Science Foundation of China[91854104] ; National Natural Science Foundation of China[31690103] ; High Level Medicine Foundation of Shanghai Government ; Shanghai Municipal Science and Technology Major Project[2017SHZDZX01]
WOS关键词	LIQUID PHASE-SEPARATION ; STRESS GRANULES ; LIVE-CELL ; PROTEIN ; GROWTH ; DNA ; TRANSITION ; MICROSCOPY ; SECRETION ; RESISTANT
WOS研究方向	Cell Biology ; Developmental Biology
语种	英语
WOS记录号	WOS:000706446300008
资助机构	Tsinghua University ; National Key R&D Program of China ; National Natural Science Foundation of China ; High Level Medicine Foundation of Shanghai Government ; Shanghai Municipal Science and Technology Major Project
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/87623]
专题	力学研究所_国家微重力实验室
通讯作者	Chen, Feng-Jung; Li, Peng
作者单位	1.Shanghai Qi Zhi Inst, Shanghai 200030, Peoples R China 2.Peking Univ, Coll Future Technol, Inst Mol Med, Beijing 100101, Peoples R China 3.Peking Univ, Coll Future Technol, Ctr Life Sci, State Key Lab Membrane Biol, Beijing 100101, Peoples R China 4.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China 5.Chinese Acad Sci, Inst Mech, 15 Beisihuanxi Rd, Beijing 100190, Peoples R China 6.Natl Univ Singapore, NUS Ctr Bioimaging Sci, 14 Sci Dr 4, Singapore 117557, Singapore 7.Natl Univ Singapore, Dept Chem, 14 Sci Dr 4, Singapore 117557, Singapore 8.Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117557, Singapore 9.Peking Univ, Coll Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China 10.Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Sch Life Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Lyu, Xuchao,Wang, Jia,Wang, Jianqin,et al. A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion[J]. DEVELOPMENTAL CELL,2021,56(18):2592-+.
APA	Lyu, Xuchao.,Wang, Jia.,Wang, Jianqin.,Yin, Ye-Sheng.,Zhu, Yun.,...&Li, Peng.(2021).A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion.DEVELOPMENTAL CELL,56(18),2592-+.
MLA	Lyu, Xuchao,et al."A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion".DEVELOPMENTAL CELL 56.18(2021):2592-+.