Bacterially self-assembled encapsulin nanocompartment for removing silver from water

doi:10.1016/j.watres.2020.116800

	Bacterially self-assembled encapsulin nanocompartment for removing silver from water
	Xing, Chong-Yang 1,3; Ma, Teng-Fei 2; Guo, Jin-Song 4; Shen, Yu 2; Yan, Peng 4; Fang, Fang 4; Chen, You-Peng 1,3,4
刊名	WATER RESEARCH
	2021-03-01
卷号	191 页码:10
关键词	Protein cage Self-assembly Bioremediation Silver Proteomics
ISSN号	0043-1354
DOI	10.1016/j.watres.2020.116800
通讯作者	Chen, You-Peng(ypchen@cqu.edu.cn)
英文摘要	Compartmentalization can protect cells from the interference of external toxic substances by sequestering toxic products. We hypothesized that proteinaceous nanocompartments may be a feasible candidate material to be added to genetically modified bacteria for the sequestration of toxic environmental products, which would open up a new bioremediation pathway. Here, we showed that the model bacterium (Escherichia coli) with self-assembling nanocompartments can remove silver (Ag) from water. Transmission electron microscopy and energy dispersive X-ray (TEM-EDX) analysis showed that the nanocompartments combined stably with silver in vitro . In addition, when exposed to 30 mu M AgNO3, the survival rate of genetically modified bacteria (with nanocompartments) was 86%, while it was just 59% in the wild-type bacteria (without nanocompartments). Label-free quantitative proteomics indicated that the nanocompartments enhanced bacterial activity by inducing the up-regulation of protein processing and secondary metabolites, and decreased their intracellular silver concentration, both of which contributed to their increased resistance to toxic silver. This study on nanocompartments has contributed to a deeper understanding of how bacteria respond to environmental stressors like heavy metal pollutants in water. The technology promises to provide a new strategy for recycling heavy metals from sewage. (c) 2020 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[21876016] ; National Natural Science Foundation of China[52070031] ; Chongqing Science and Technology Bureau[cstc2018jcyjAX0366] ; Chongqing Science and Technology Bureau[cstc2018jcyjAX0638]
WOS研究方向	Engineering ; Environmental Sciences & Ecology ; Water Resources
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000615940000005
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/13041]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Chen, You-Peng
作者单位	1.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 2.Chongqing Technol & Business Univ, Natl Base Int Sci & Technol Cooperat Intelligent, Chongqing 400067, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligence Technol, Key Lab Reservoir Aquat Environm, Chongqing 400714, Peoples R China 4.Chongqing Univ, MOE, Key Lab Three Gorges Reservoir Reg Ecoenvironm, Chongqing 400045, Peoples R China
推荐引用方式 GB/T 7714	Xing, Chong-Yang,Ma, Teng-Fei,Guo, Jin-Song,et al. Bacterially self-assembled encapsulin nanocompartment for removing silver from water[J]. WATER RESEARCH,2021,191:10.
APA	Xing, Chong-Yang.,Ma, Teng-Fei.,Guo, Jin-Song.,Shen, Yu.,Yan, Peng.,...&Chen, You-Peng.(2021).Bacterially self-assembled encapsulin nanocompartment for removing silver from water.WATER RESEARCH,191,10.
MLA	Xing, Chong-Yang,et al."Bacterially self-assembled encapsulin nanocompartment for removing silver from water".WATER RESEARCH 191(2021):10.