Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore

doi:10.3390/s21010033

	Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore
	Yan, Han 2; Zhang, Zhen 2; Weng, Ting 1; Zhu, Libo 2; Zhang, Pang 1; Wang, Deqiang 1; Liu, Quanjun 2
刊名	SENSORS
	2021
卷号	21 期号:1 页码:11
关键词	nanopore DNA logic gate DNA tetrahedron probe AND operation
DOI	10.3390/s21010033
通讯作者	Liu, Quanjun(lqj@seu.edu.cn)
英文摘要	Nanopores have a unique advantage for detecting biomolecules in a label-free fashion, such as DNA that can be synthesized into specific structures to perform computations. This method has been considered for the detection of diseased molecules. Here, we propose a novel marker molecule detection method based on DNA logic gate by deciphering a variable DNA tetrahedron structure using a nanopore. We designed two types of probes containing a tetrahedron and a single-strand DNA tail which paired with different parts of the target molecule. In the presence of the target, the two probes formed a double tetrahedron structure. As translocation of the single and the double tetrahedron structures under bias voltage produced different blockage signals, the events could be assigned into four different operations, i.e., (0, 0), (0, 1), (1, 0), (1, 1), according to the predefined structure by logic gate. The pattern signal produced by the AND operation is obviously different from the signal of the other three operations. This pattern recognition method has been differentiated from simple detection methods based on DNA self-assembly and nanopore technologies.
资助项目	National Natural Science Foundation of China[61827814] ; National Natural Science Foundation of China[31872726] ; National Key R&D Program of Chain[2017YFC0906503] ; National Key R&D Program of Chain[2016YFA0501600] ; andWest Light Foundation of CAS
WOS研究方向	Chemistry ; Engineering ; Instruments & Instrumentation
语种	英语
出版者	MDPI
WOS记录号	WOS:000606130500001
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/12698]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Liu, Quanjun
作者单位	1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, 2,Sipailou, Nanjing 210096, Peoples R China
推荐引用方式 GB/T 7714	Yan, Han,Zhang, Zhen,Weng, Ting,et al. Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore[J]. SENSORS,2021,21(1):11.
APA	Yan, Han.,Zhang, Zhen.,Weng, Ting.,Zhu, Libo.,Zhang, Pang.,...&Liu, Quanjun.(2021).Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore.SENSORS,21(1),11.
MLA	Yan, Han,et al."Recognition of Bimolecular Logic Operation Pattern Based on a Solid-State Nanopore".SENSORS 21.1(2021):11.