The role of morphological changes in algae adaptation to nutrient stress at the single-cell level

doi:10.1016/j.scitotenv.2020.142076

	The role of morphological changes in algae adaptation to nutrient stress at the single-cell level
	Yan, Peng 2; Guo, Jin-song 2; Zhang, Ping 3; Xiao, Yan 4; Li, Zhe 4; Zhang, Shu-qing 2; Zhang, Yu-xin 1; He, Shi-xuan 4
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2021-02-01
卷号	754 页码:11
关键词	Algae Single-cell Nutrient stress Morphology Physiology Adaptation
ISSN号	0048-9697
DOI	10.1016/j.scitotenv.2020.142076
通讯作者	Guo, Jin-song(guo0768@cqu.edu.cn)
英文摘要	Individual cell heterogeneity within a population can be critical to its peculiar function and fate. Conventional algal cell-based assays mainly analyze the average responses from a population of algal cells. Therefore, the mechanisms through which changes in population characteristics are driven by the behavior of single algal cells are still not well understood. Algal cells may modulate their physiology and metabolism by changing their morphology in response to environmental stress. In this study, an algal single-cell culture and analysis system was developed to investigate the potential role of morphological changes by algal cells during adaptation to nutrient stress based on a microwell array chip. The surface-to-volume ratio of Microcystis aeruginosa (M. aeruginosa) and the volume of Scenedesmus obliquus (S. obliquus) significantly increased with increasing culture time under nutrient stress. The eccentricity of M. aeruginosa and S. obliquus gradually increased and decreased, respectively, with increasing culture time, indicating that the morphology of M. aeruginosa and S. obliquus became increasingly irregular and regular, respectively, under nutrient stress. There were significant correlations between the morphological characteristics and physiological characteristics of M. aeruginosa and S. obliquus under nutrient stress. In M. aeruginosa, an increased surface-to-volume ratio facilitated a high specific fluorescence intensity, specific Raman intensity, and maximum electron transport rate. In S. obliquus, increased cell volume enhanced nutrient absorption, which facilitated a higher specific growth rate. M. aeruginosa and S. obliquus adopted different adaptation strategies in response to nutrient stress based on morphological changes. These findings facilitate the development of management strategies for controlling harmful cyanobacterial blooms. (C) 2020 Elsevier B.V. All rights reserved.
资助项目	National Key Research and Development Program of China[2019YFD1100200] ; Chongqing Science and Technology Bureau[cstc2018jcyjAX0610] ; Fundamental Research Funds for the Central Universities[2019CDQYCH036] ; Fundamental Research Funds for the Central Universities[2020CDJXZ001]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000593899600007
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/12612]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Guo, Jin-song
作者单位	1.Chongqing Univ, Coll Mat Sci & Engn, Chongqing 400044, Peoples R China 2.Chongqing Univ, Coll Environm & Ecol, Chongqing 400045, Peoples R China 3.Guizhou Minzu Univ, Coll Ecoenvironm Engn, Guiyang 550025, Guizhou, Peoples R China 4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm CAS, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Yan, Peng,Guo, Jin-song,Zhang, Ping,et al. The role of morphological changes in algae adaptation to nutrient stress at the single-cell level[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,754:11.
APA	Yan, Peng.,Guo, Jin-song.,Zhang, Ping.,Xiao, Yan.,Li, Zhe.,...&He, Shi-xuan.(2021).The role of morphological changes in algae adaptation to nutrient stress at the single-cell level.SCIENCE OF THE TOTAL ENVIRONMENT,754,11.
MLA	Yan, Peng,et al."The role of morphological changes in algae adaptation to nutrient stress at the single-cell level".SCIENCE OF THE TOTAL ENVIRONMENT 754(2021):11.