Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy

doi:10.1002/jbio.202000315

	Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy
	Tang, Mingjie 1,2; Zhang, Mingkun 2; Xia, Liangping 2,4; Wei, Dongshan 2,3; Yang, Zhongbo 2; Yan, Shihan 2; Du, Chunlei 2; Cui, Hong-Liang 2
刊名	JOURNAL OF BIOPHOTONICS
	2020-10-11
页码	10
关键词	ATR DNA oligonucleotides microfluidic cell repeated sequence terahertz absorption
ISSN号	1864-063X
DOI	10.1002/jbio.202000315
通讯作者	Xia, Liangping(xialp@yznu.edu.cn) ; Cui, Hong-Liang(hcui@cigit.ac.cn)
英文摘要	Terahertz absorption spectroscopy based on attenuated total reflection (ATR) from a microfluidic sample cell was designed and implemented to detect gene mutations leading to Huntington's disease (HD). The self-developed compact ATR microfluidic system was employed to detect two groups of base-repeated DNA molecules combined with a terahertz time-domain spectrometer in a marker-free manner. The first group featured different repetition patterns of oligonucleotide fragments, and the second group included the HD gene. For the oligonucleotides of different repetition patterns, there were significant differences among the three oligonucleotides with three repeats of the double bases, which could be unambiguously classified and identified; For the HD gene, it was found that the magnitude of the terahertz absorption coefficients of the four oligonucleotide solutions was, in ascending order, CAG-4 < CAG-16 < CAG-32 < CAG-40 (the numbers are the repeat times of the CAG base segment, with 40 repeats belonging to the HD gene), when the concentration of oligonucleotide was 1 mg/mL. Principal component analysis result indicated that the spectral differences of the four oligonucleotide solutions with different CAG repeat times were statistically significant and clearly distinguishable. These results demonstrate the potential of terahertz spectroscopy as a noninvasive, unmarked, fast and low-cost assay for gene diagnosis and clinical disease detection.
资助项目	Central Government Supported Key Instrument Program of China[YXGYQ201700136] ; National Key Research and Development Program of China[2017YFF0106303] ; National Natural Science Foundation of China[61775213 61875196] ; Natural Science Foundation of Chongqing[cstc2019jcyj-msxmX0654 cstc2019jcyj-msxmX0352]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Optics
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000578419600001
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/11889]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Xia, Liangping; Cui, Hong-Liang
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 3.Dongguan Univ Technol, Sch Elect Engn, Dongguan, Peoples R China 4.Yangtze Normal Univ, Key Lab Micro Nano Optoelect Devices & Intelligen, Chongqing, Peoples R China
推荐引用方式 GB/T 7714	Tang, Mingjie,Zhang, Mingkun,Xia, Liangping,et al. Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy[J]. JOURNAL OF BIOPHOTONICS,2020:10.
APA	Tang, Mingjie.,Zhang, Mingkun.,Xia, Liangping.,Wei, Dongshan.,Yang, Zhongbo.,...&Cui, Hong-Liang.(2020).Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy.JOURNAL OF BIOPHOTONICS,10.
MLA	Tang, Mingjie,et al."Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy".JOURNAL OF BIOPHOTONICS (2020):10.