Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum

doi:10.1007/s00284-020-01961-0

	Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum
	Su, HaiFeng 3,4; Chen, Hua 2; Lin, JiaFu 1
刊名	CURRENT MICROBIOLOGY
	2020-04-16
页码	8
ISSN号	0343-8651
DOI	10.1007/s00284-020-01961-0
通讯作者	Lin, JiaFu(linjiafu@cdu.edu.cn)
英文摘要	Non-natural 2-methyl-1-butanol (2 MB) has been biosynthesized through the modification of metabolic pathways using Corynebacterium crenatum, a non-model host. However, its production capacity is not effectively improved. In this study, the fermentation process was strengthened through factor combination design (FCD) for enhancing the production of 2 MB. Our results showed that the highest production of 2 MB, 3-methyl-1-butanol (3 MB), ethanol, and total solvent was 4.87 +/- 0.39 g/L, 3.57 +/- 0.21 g/L, 5.74 +/- 0.43 g/L, and 14.18 g/L, respectively, under the optimal fermentation conditions. The optimal fermentation conditions were determined through the FCD to be as follows: pH of 6.5, IPTG concentration of 1.2 mM, fermentation temperature of 32 degrees C, and fermentation time of 96 h. This study provides a significant guidance for the optimal control technology of the genetically engineered C. crenatum, and also a useful reference for the industrial production of 2 MB via the microbial fermentation approach.
资助项目	Open Project Program of the State Key Laboratory of Petroleum Pollution Control[PPC2017004] ; CNPC Research Institute of Safety and Environmental Technology ; National Natural Science Foundation[cstc2017jcyjA02138] ; frontier research program of Chongqing[cstc2017jcyjA02138] ; Chongqing Science Committee, Key laboratory of Degraded and Unused Land Consolidation Engineering, the Ministry of Natural and Resources[SXDJ2019]
WOS研究方向	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000533859200004
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/11019]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Lin, JiaFu
作者单位	1.Chengdu Univ, Sichuan Ind Inst Antibiot, Antibiot Res & Re Evaluat Key Lab Sichuan Prov, Chengdu, Peoples R China 2.Zunyi Inst Forestry Sience, Zunyi 563002, Guizhou, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Water Environm, 266 Fangzheng Ave,Shuitu High Tech Pk, Chongqing 400714, Peoples R China 4.State Key Lab Petr Pollut Control, Beijing 102206, Peoples R China
推荐引用方式 GB/T 7714	Su, HaiFeng,Chen, Hua,Lin, JiaFu. Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum[J]. CURRENT MICROBIOLOGY,2020:8.
APA	Su, HaiFeng,Chen, Hua,&Lin, JiaFu.(2020).Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum.CURRENT MICROBIOLOGY,8.
MLA	Su, HaiFeng,et al."Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum".CURRENT MICROBIOLOGY (2020):8.